LMH Chicken Liver Hepatocellular carcinoma cell line鸡肝癌细胞株 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

LMH Chicken Liver Hepatocellular carcinoma cell line鸡肝癌细胞株

BioVector NTCC中国质粒载体菌种细胞基因保藏中心

LMH Chicken Liver Hepatocellular carcinoma cell line

Cat No.: NTCC594220

Organism: Gallus gallus (chicken)

Strain: Leghorn

Tissue: Liver

Morphology: Epithelial; Dendritic like.

Celltype: hepatocellular carcinoma; induced using diethylnitrosamine.

Growth Properties: adherent

Description: The cell line LMH was established in 1981 by Tomoyuki Kitagawa (see reference below) at the Cancer Institute, Kami-Ikebukuro-ku of

Tokyo, Japan. Primary hepatocellular carcinoma epithelial cell lines were transformed into permanent cells by inducing tumorous nodules by long

term treatment with diethylnitrosamine in the liver of a male leghorn cchicken. LMH cells are inducible for the expression of the liver specific

apolipoprotein II (apoll) gene.

Karyotype triploid; modal number = 116; six marker chromosomes

Derivation LMH is a primary hepatocellular carcinoma epithelial cell line

established in 1981 by Tomoyuki Kitagawa at the Cancer Institute,

Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.

Clinical Data Tumorous nodules were induced in the liver of a male leghorn chicken

by long term treatment with diethylnitrosamine.

Receptor Expression estrogen receptor, positive

Tumorigenic Yes

Effects Yes, forms tumors in nude mice

Comments Tumorous nodules were induced in the liver of a male leghorn chicken

by long term treatment with diethylnitrosamine.

The morphology of the cells is dendritic like.

The cells express glucose-6-phosphatase and weak canalicular

ATPase activity.

The cells form tumors in athymic nude mice.

LMH cells contain a low level of functional estrogen receptor and are

inducible for the expression of the liver specific apolipoprotein II (apoII)

gene.

The cell line is useful for transfection studies.

NOTE: tissue culture vessels used to propagate this line must be

precoated with 0.1% gelatin.

To prepare the vessels, flood them with gelatin solution, refrigerate for

5 to 10 minutes and remove the liquid.

Vessels prepared in this fashion may be used immediately.

Complete Growth Medium 89%1640+10%FBS+ 100 U/ml penicillin G sodium (option); 100 μ g/ml

streptomycin sulfate (option);

or

Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%

Subculturing Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse two times with cold 0.25% trypsin, 0.03%

EDTA solution.

Allow the flasks to sit at room temperature (or incubate at 37° C) until

the cells detach.

Add fresh culture medium, aspirate and dispense into new culture

flasks.

Flasks must be coated with 0.1% gelatin.

Cryopreservation Culture medium, 95%; DMSO, 5%

References Binder R, et al. Expression of endogenous and transfected

apolipoprotein II and vitellogenin II genes in an estrogen responsive

chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990.

PubMed:2330000

Kawaguchi T, et al. Establishment and characterization of a chicken

hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463,

1987. PubMed: 3607775