• Vector backbone
  pAAV
  (Search Vector Database)
• Vector type
  Mammalian Expression, AAV, CRISPR

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

GENE/INSERT 1

• Gene/Insert name
  hSaCas9
• Species
  Staphylococcus aureus
• Insert Size (bp)
  3345
• GenBank ID
  CCK74173.1

• Tags / Fusion Proteins

  • NLS (N terminal on insert)

  • NLS (C terminal on insert)

  • HA (C terminal on insert)

  • OLLAS tag (C terminal on insert)

  
  

GENE/INSERT 2

• Gene/Insert name
  Chimeric guide for SaCas9
• Species
  Staphylococcus aureus
• Insert Size (bp)
  101