pOET1N-6xHis昆虫杆状病毒表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pOET1N-6xHis昆虫杆状病毒表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Plasmid Description:

pOET1N is a baculovirus transfer vector designed for high level expression of foreign genes under thepowerful AcMNPV polyhedron (polh) promoter. The vector encodes an optional N-terminal 6×His-Tag®fusionsequence that may be utilized if the insert allows read-through in the correct reading frame. This greatlyeases the purification of the recombinant protein since the 6×His-containing fusion proteins bind with highaffinity to Ni-NTA Agarose. If required, the 6×His-Tag®can be removed by incubating the fusion protein in thepresence of the proteinase cleavage enzyme Thrombin. pOET1N is smaller than other available transfervectors (4598 bp) which greatly facilitate the cloning steps. It has a Col E1 origin of replication and anampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloningsite (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation, asshown on the circular map. The coding strand of the MCS as transcribed from the polh promoter is shownbelow the circular map. The PacI site at the end of the MCS provides translational stop codons in all threereading frames for expression of truncated proteins. The AcMNPV sequences flanking the gene in the transfervectors MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus.pOET1N is compatible with any baculovirus system that utilizes homologous recombination in insect cells.

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