逆转录病毒包装细胞株RetroPack PT67 , GP2-293, AmphoPack-293, EcoPack2-293, cell line-BioVector NTCC Inc.

逆转录病毒包装细胞株RetroPack PT67 , GP2-293, AmphoPack-293, EcoPack2-293, cell line

-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Retro-X Retroviral Packaging Cell Lines

To produce recombinant retrovirus for target cell infection, retroviral transfer vectors must be transfected intoretroviral packaging cells that provide the gag, pol, and env genes in trans. Various options are available, andall retroviral packaging cell lines and systems are compatible with all retroviral transfer vectors sold byClontech, including MMLV- and MSCV-based retroviruses. Table 1 summarizes the retroviral packaging celllines available from Clontech and Table 2 lists the host range of retroviruses created with the different types ofcommonly used envelopes.

The RetroPack PT67 Cell Line is the recommended packaging cell line for creatinga stable producer cell line for continuous production of retrovirus. This retroviral packaging systemincludes an NIH/3T3-based packaging cell line that expresses the 10A1 viral envelope. Virus packagedusing RetroPack PT67 cells can be used to infect a broad range of mammalian cells, including human,mouse and rat cell types. The virus is able to enter cells via two different surface molecules, theamphotropic retrovirus receptor (Ram-1) and the GALV receptor.NOTE: If you wish to make retrovirus via transient transfection, which is the preferred method formost researchers, we recommend the Retro-X Universal Packaging System, or the AmphoPack 293 orEcoPack 2-293 Cell Lines.  

The Retro-X Universal Packaging System is our premium retroviral packagingsystem featuring the GP2-293 packaging cell line. All four commonly used envelopes are supplied onseparate vectors that you cotransfect with your transfer vector. Envelope choices include VSV-G, eco,ampho, and 10A1 to allow you to choose the tropism that is most appropriate for your target cells (seeTable 2).

The EcoPack™ 2-293 Cell Line is an ecotropic HEK 293-based packaging cellline that is also easy to transfect and produces retrovirus with a tropism that limits infection to mouseand rat cells*. The viral envelope protein is integrated and expressed by EcoPack 2-293, andrecognizes the ecotropic receptor (mCAT1) found only on the surface of these cell types.*NOTE: It is possible to transduce human cells with ecotropic retrovirus provided that the cells arepretreated with Ecotropic Receptor Booster (Cat. No. 631471; Section III.J). Ecotropic ReceptorBooster consists of a vial of concentrated exosome-like vesicles that are densely coated with themCAT-1 receptor protein. When the booster vesicles are applied to your cells, they fuse with theplasma membrane, resulting in an increased level of the receptor on the cell surface.

The AmphoPack-293 Cell Line can be used to produce recombinant MMLV-basedviral particles that infect a broad range of mammalian cells, including human, mouse, and rat cells.AmphoPack 293 is also derived from an HEK 293-based cell line that is easily transfected to producehigh viral titers. The viral envelope protein recognizes the amphotropic receptor (Ram-1).

The Pantropic Retroviral Expression System also features the GP2-293packaging cell line, which expresses gag and pol proteins. The envelope protein is supplied on aseparate plasmid, pVSV-G, which is cotransfected with your transfer vector. Delivery using a VSV-Genvelope is not dependent on a cell surface receptor; instead, it mediates viral entry through lipidbinding and plasma membrane fusion. Two transfer expression vectors are included with the PantropicSystem, giving you the choice of using a retroviral 5' LTR or the Drosophila hsp70 promoter toexpress your gene of interest.