PX552腺相关病毒CRISPR/Cas9基因编辑载体Plasmid 60958-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

价　　格：¥4950
货　　号：PX552 vector
产　　地：北京

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PX552
(BioVector-060958)

Map图谱：

PURPOSE

pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

Vector backbone
pAAV
(Search Vector Database)
Backbone size w/o insert (bp)2905
Total vector size (bp)5314
Vector type
Mammalian Expression, Mouse Targeting, AAV, CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
Stbl3
Copy number
High Copy

GENE/INSERT 1

Gene/Insert name
U6_(SpaI)_sgRNA
Species
Synthetic
Insert Size (bp)
351
PromoterU6

CLONING INFORMATION FOR GENE/INSERT 1

Cloning methodGibson Cloning
5′ sequencing primercgcgtgagggcctatttcc
3′ sequencing primergcaagtgggttttaggaccag

(Common Sequencing Primers)

GENE/INSERT 2

Gene/Insert name
Syn_EGFP-KASH
Species
Synthetic
Insert Size (bp)
349
PromoterSynapsin

CLONING INFORMATION FOR GENE/INSERT 2

Cloning methodGibson Cloning
5′ sequencing primerctgcgtatgagtgcaagtgggtt
3′ sequencing primerccgttggtgtaccgcagcatg

Sequence序列：

COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|614088139|
COMMENT     VNTNAME|pAAV-hSyn-hChR2(H13R)-EYFP|
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /dnas_title="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     polyA_signal    2048..2526
                     /dnas_title="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     misc_feature    2566..2706
                     /dnas_title="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2798..3104
                     /dnas_title="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     misc_feature    3623..4480
                     /dnas_title="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4631..5298
                     /dnas_title="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     gene            1017..1733
                     /gene="EGFP"
                     /vntifkey="60"
     CDS             1752..2000
                     /dnas_title="KASH domain of Nesprin2"
                     /vntifkey="4"
                     /label=KASH domain of Nesprin2
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    405..504
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    415..421
                     /note="SapI"
     misc_feature    406..412
                     /note="SapI"
     misc_feature    402..404
                     /note="5'"
     misc_feature    423..425
                     /note="3'"
     misc_feature    505..986
                     /note="hSyn"
     misc_feature    151..155
                     /note="MluI"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
        181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
        241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
        301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
        361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCggaaga gcgagctctt
        421 ctgttttaga gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa
        481 gtggcaccga gtcggtgcTT TTTTtctaga ctgcagaggg ccctgcgtat gagtgcaagt
        541 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
        601 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
        661 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
        721 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
        781 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
        841 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
        901 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
        961 gtcgtgcctg agagcgcagt cgagaaggta ccggatcctc tagagtcgac gccaccatgg
       1021 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
       1081 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
       1141 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
       1201 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
       1261 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
       1321 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
       1381 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       1441 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
       1501 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
       1561 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
       1621 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
       1681 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
       1741 tcagatctcg agaggaggag gaggagacag acagcaggat gccccacctc gacagccccg
       1801 gcagctccca gccgagacgc tccttcctct caagggtgat cagggcagcg ctaccgttgc
       1861 agctgcttct gctgctgctg ctgctcctgg cctgcctgct acctgcctct gaagatgact
       1921 acagctgcac ccaggccaac aactttgccc gatccttcta ccccatgctg cggtacacca
       1981 acgggccacc tcccacctag gaattcgata tcaagcttat cgataccgag cgctgctcga
       2041 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
       2101 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
       2161 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
       2221 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
       2281 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
       2341 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
       2401 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
       2461 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
       2521 gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga
       2581 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
       2641 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
       2701 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
       2761 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
       2821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
       2881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
       2941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
       3001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
       3061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
       3121 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
       3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
       3241 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
       3301 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
       3361 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
       3421 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
       3481 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
       3541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
       3601 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
       3661 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
       3721 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
       3781 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
       3841 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
       3901 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
       3961 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
       4021 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
       4081 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
       4141 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
       4201 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
       4261 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
       4321 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
       4381 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
       4441 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
       4501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
       4561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
       4621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
       4681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
       4741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
       4801 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
       4861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
       4921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
       4981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
       5041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
       5101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
       5161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
       5221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
       5281 ttgctggcct tttgctcaca tgt