HepaRG cells人体肝前体细胞系终端分化肝细胞BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

HepaRG cells

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Background

HepaRG™ cells are a human hepatic progenitor cell line that retains many characteristics of primary human

hepatocytes. HepaRG™ cells are terminally differentiated and provided in a convenient cryopreserved format.

For scientists who need reproducible metabolism data, HepaRG™ cells are an in vitro tool that provides

reproducible results in a metabolically complete and scalable system.

This description and user guide for the thawing and culture of cryopreserved HepaRG™ cells includes three

sections:

 Section 1: Recommended materials, media and cells

 Section 2: Protocol for the thawing, seeding and maintenance of HepaRG™ cells

 Section 3: Cell morphology

Section 1: Materials, media and cells

Materials

 Water bath at +37° C

 Laminar flow hood

 Pipet-aid, pipettes and micropipettes

 Multichannel pipettes and repeater pipette

 Polystyrene round-bottom tubes (40mL) and petri dishes (92 x 17 mm) or similar containers

 Incubator at +37° C with a 5%/95% CO2/O2 atmosphere and 100% relative humidity

 Phase-contrast microscope

 Material for cell count (cell counting chamber, coverslips, 0.05% Trypan blue solution)

Media supplements

 Working medium is prepared by adding the HepaRG™ Supplement to 100 ml/500 ml of Williams’

Medium E and 1ml/5ml of GlutaMAX-I.

Cells

 Immediately place the cryovial(s) in liquid nitrogen upon receipt.

Section 2: Protocol

Note: Observe universal precautions when handling HepaRG™ cells and treat all biologic material as

potentially infectious.

The following steps must be performed under a laminar flow hood.