- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
240e兔骨髓瘤样细胞株
BioVector NTCC中国质粒载体菌种细胞基因保藏中心
Cell line name 240E
Comments
Group: Hybridoma fusion partner cell line.
Selected for resistance to: 8-azaguanine.
Disease
Rabbit plasmacytoma
Species of origin Oryctolagus cuniculus (Rabbit)
Hierarchy
Parent: (240E-W)
Category
Cancer cell line
Publications
Patent=US7429487
Pytela R., Zhu W., Ke Y., Qian Q., Au H.C.
Fusion partner for production of monoclonal rabbit antibodies.
Patent number US7429487, 30-Sep-2008
240E-W2 cell line doesn't carry an endogenous heavy chain gene.
Cell Fusion
Fusions were performed using conventional methodology. 1.5−3×108 lymphocytes from an immunized rabbit
and the fusion partner cells were mixed at a ratio of 2:1 with PEG 4000 at (Sigma P7181) 37° C. in serum-free
medium. The cells were transferred into 96-well cell culture plates at approximately 1×105 lymphocytes per well
in 1640 RPMI medium with 15% FBS (or FCS), incubated in a CO2 incubator. After 48 hr HAT medium (Sigma
H0262) was added. Hybridoma colonies were ready for screening in 3-5 weeks. Medium was changed 2-3
times before screening by removing old medium and add fresh medium. Supernatants were tested for the
presence of antibody, specific for the immunogen, by ELISA. Western blot analysis was used as a secondary
screening assay. The hybridomas were sub-cloned by limit dilution. The fusion partner 240E-W2 at 2×104 cells
per well was used as feeder cells.
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
www.biovector.net
您正在向 biovector.net 发送关于产品 240e兔骨髓瘤样细胞株 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 的询问
- 公告/新闻
