MPRO Cell Line, Clone 2.1 ATCC CRL-11422 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

MPRO Cell Line, Clone 2.1 ATCC CRL-11422 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 运输方式： 冻存运输 细胞类型： 其他细胞类型 是否是肿瘤细胞： 0 物种来源： 小鼠 数量： 大量 器官来源： 骨髓 ATCC Number： CRL-11422™ 生长状态： 悬浮生长 品系： BDF1 Designations: MPRO Cell Line, Clone 2.1 Depositors: Fred Hutchinson Cancer Res. Cntr. Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ] Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Mus musculus deposited as mouse Morphology: Source: Organ: bone marrow Strain: BDF1 Cell Type: infected with the retroviral vector LRARalpha403SN Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Gender: male Comments: Bone marrow cells of male BDF1 mice were infected with the retroviral vector LRARalpha403SN. RARalpha403 was inserted into the retroviral vector LXSN to generate the recombinant vector designated LRARalpha403SN. RARalpha403 is a truncated cDNA that encodes a peptide of 403 amino acids containing the N-terminus and the DNA-binding domain. RARalphacDNA contains a truncation of the sequences coding for the C-terminal 59 amino acids as well as a portion of the 3' untranslated region. The infected cells were subsequently selected in a medium containing G418 and GM-CSF. The infected cells proliferated and stayed immature in morphology. This GM-CSF dependent neutrophil progenitor cell line is capable of differentiating into neutrophils but not monocytes, mast cells or basophils. The cells exhibit promyelocyte characteristics including the presence of numerous azurophilic primary granules and cell surface epression of mouse neutrophIL-specific antigen 7/4. They exhibited positive staining for chloroacetate esterase. Northern blot analysis indicates that the MPRO cells express high levels of retroviral mRNA harboring the truncated RARalpha403 sequence. Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate containing 10 ng/ml murine granulocyte macrophage colony stimulating factor, 80%; heat-inactivated horse serum, 20% Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml. Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 References: 39126: Weiler SR, et al. D3: a gene induced during myeloid cell differentiation of Linlo c-Kit+ Sca-1(+) progenitor cells. Blood 93: 527-536, 1999. PubMed: 9885214 45099: Tsai S, Collins SJ. Creating novel hematopoietic cell lines by expressing altered retinoic acid receptors. US Patent 5,830,760 dated Nov 3 1998 48261: Scott LM, et al. E3, a hematopoietic-specific transcript directly regulated by the retinoic acid receptor alpha. Blood 88: 2517-2530, 1996. PubMed: 8839844 49678: Johnson BS, et al. Retinoid X receptor (RXR) agonist-induced activation of dominant-negative RXR-retinoic acid receptor alpha403 heterodimers is developmentally regulated during myeloid differentiation. Mol. Cell. Biol. 19: 3372-3382, 1999. PubMed: 10207061 49679: Du Y, et al. Identification of an interleukin-3-regulated aldoketo reductase gene in myeloid cells which may function in autocrine regulation of myelopoiesis. J. Biol. Chem. 275: 6724-6732, 2000. PubMed: 10702227 49683: Lawson ND, et al. Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines. Exp. Hematol. 26: 1178-1185, 1998. PubMed: 9808058 49684: Lawson ND, et al. Isolation and characterization of the cDNA for mouse neutrophil collagenase: demonstration of shared negative regulatory pathways for neutrophil secondary granule protein gene expression. Blood 91: 2517-2524, 1998. PubMed: 9516153