pTT5哺乳动物细胞瞬时高表达载体 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

LOCUS       pTT5            4401 bp    DNA     circular      8-NOV-2015
DEFINITION  pTT5 vector.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTIhttp://www.biovector.net/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="pTT5"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        43..619
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     enhancer        42..421
                     /vntifkey="9"
                     /label=CMV\enhancer
     promoter        565..646
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    576..596
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     misc_feature    620..639
                     /vntifkey="21"
                     /label=pCEP_fwd_primer
     misc_feature    622..646
                     /vntifkey="21"
                     /label=LNCX_primer
     promoter        2536..2564
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            2606..3466
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             2606..3466
                     /vntifkey="4"
                     /label=Amp
                     /note="Amp resistant gene"
     rep_origin      3621..4240
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    1165..1256
                     /vntifkey="21"
                     /label=MCS
BASE COUNT     1099 a      1036 c      1087 g      1179 t 
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag 
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt 
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg 
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg 
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc 
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg 
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt 
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt 
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg 
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa 
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc 
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc 
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagaggg 
     1201 cccgtttctg ctagcaagct tgctagcggc cgctcgaggc cggcaaggcc ggatcccccg 
     1261 acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 
     1321 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga gatccctcgg 
     1381 agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct 
     1441 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca 
     1501 actgaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa 
     1561 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt 
     1621 aaaagggtcc taaggaacag cgatgtaggt gggcgggcca agataggggc gcgattgctg 
     1681 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc 
     1741 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac 
     1801 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc 
     1861 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc 
     1921 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc 
     1981 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc 
     2041 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc 
     2101 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa 
     2161 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa 
     2221 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa 
     2281 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa 
     2341 tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga gaattaattc 
     2401 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 
     2461 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     2521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     2581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     2641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     2701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     2761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     2821 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg 
     2881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     2941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     3001 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     3061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     3121 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt 
     3181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     3241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     3301 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     3361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     3421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     3481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     3541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     3601 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     3661 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     3721 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     3781 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     3841 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     3901 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     3961 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     4021 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     4081 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     4141 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     4201 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     4261 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     4321 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     4381 cgagtcagtg agcgaggaag c 
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