| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pREP7 |
| 质粒类型: | 哺乳动物表达载体；附加体载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | RSV |
| 载体大小: | 9494 bp |
| 5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | DH5α等 |
| 宿主细胞（系）: | 人类细胞；灵长类细胞；犬类细胞 |
| 备注: | pREP7载体是附加体型载体，可以在基因组外自主复制，类似的载体有： pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
pREP7载体
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pREP7 9494 bp DNA SYN
DEFINITION pREP7
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9494
/organism="pREP7"
/mol_type="other DNA"
misc_feature 583..601
/label="pREP_fwd_primer"
misc_feature complement(717..736)
/label="EBV_rev_primer"
misc_feature complement(969..991)
/label="pGEX_3_primer"
CDS 1084..1818
/label="ORF frame 1"
/translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN
HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH
PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR
VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV
PAPDRTIASHRPCAQAASSKLPSTKL*"
terminator complement(1339..1609)
/label="TK_PA_terminator"
/translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN
HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH
PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR
VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV
PAPDRTIASHRPCAQAASSKLPSTKL*"
CDS complement(1617..2642)
/label="ORF frame 1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene complement(1620..2627)
/label="hygroB"
/gene="hygroB"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
CDS 1884..3254
/label="ORF frame 3"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
promoter 2761..2909
/label="TATA_promoter"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
gene 2916..3254
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
CDS 3158..3769
/label="ORF frame 2"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
gene complement(4203..5063)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
CDS complement(4203..5063)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5105..5133)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 5804..6925
/label="ORF frame 2"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR
GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD
VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR
ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA
PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD
GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*"
rep_origin 7229..9399
/label="EBV_origin"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR
GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD
VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR
ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA
PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD
GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*"
ORIGIN
1 tctagagtcg accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg
61 gtgcactctc agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc
121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
241 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg
301 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
361 gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt
421 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
481 tggtacgatc gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc
541 actgaattcc gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc
601 catttgacca ttcaccacat tggtgtgcac ctccaagctg ggtaccagct gctagcaagc
661 ttgctagcgg ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg
721 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
781 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
841 gcattcattt tatgtttcag gttcaggggg aggtggggag gttttttaaa gcaagtaaaa
901 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
961 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
1021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
1081 gccatgaccg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
1141 gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
1201 aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca
1261 acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
1321 aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
1381 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
1441 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
1501 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
1561 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat
1621 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
1681 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
1741 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
1801 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
1861 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
1921 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
1981 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
2041 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
2101 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
2161 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
2221 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
2281 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
2341 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
2401 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
2461 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
2521 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
2581 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
2641 atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg
2701 gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc
2761 gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt
2821 gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac
2881 gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct
2941 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
3001 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
3061 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
3121 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
3181 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
3241 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
3301 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
3361 atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt
3421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
4021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
4081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
4141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
4201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
4261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
4321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
4381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
4441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
4501 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
4561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
4621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
4681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
4741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
4801 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
4861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
4921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
4981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
5041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
5161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
5221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
5281 ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
5341 ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
5401 catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
5461 acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
5521 acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
5581 aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
5641 tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
5701 gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
5761 ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
5821 agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
5881 agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
5941 acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
6001 gggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg gaggccgccg
6061 gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg
6121 tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc
6181 accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg aagccgatta
6241 ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc cgggagcgat
6301 agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc ggggtcaggg
6361 tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc aaggaggttc
6421 caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta ggagtcacgt
6481 agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa
6541 gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat gtcgtcttac
6601 accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac ctggcccgct
6661 aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat ttgctgaggt
6721 tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct gcaatatcag
6781 ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc cacctatggt
6841 ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag gtgatggaga
6901 tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca atcgtattaa
6961 aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg cgtgctgttg
7021 gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg ggcaattggg
7081 catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca
7141 ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa
7201 attcacctaa gaatgggagc aaccagcatg caggaaaagg acaagcagcg aaaattcacg
7261 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
7321 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
7381 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
7441 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
7501 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
7561 atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
7621 gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
7681 gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
7741 gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
7801 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
7861 gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
7921 cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
7981 cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
8041 gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
8101 tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
8161 acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
8221 tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
8281 agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
8341 ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
8401 ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
8461 atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
8521 atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
8581 gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
8641 attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
8701 gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
8761 attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
8821 gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
8881 gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
8941 ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
9001 ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
9061 ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
9121 cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
9181 ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
9241 gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
9301 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
9361 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gaatacaaaa
9421 caaaagcgct cctcgtacca gcgaagaagg ggcagagatg ccgtagtcag gtttagttcg
9481 tccggcggcg gggc
//
LOCUS pREP7 9494 bp DNA SYN
DEFINITION pREP7
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9494
/organism="pREP7"
/mol_type="other DNA"
misc_feature 583..601
/label="pREP_fwd_primer"
misc_feature complement(717..736)
/label="EBV_rev_primer"
misc_feature complement(969..991)
/label="pGEX_3_primer"
CDS 1084..1818
/label="ORF frame 1"
/translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN
HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH
PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR
VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV
PAPDRTIASHRPCAQAASSKLPSTKL*"
terminator complement(1339..1609)
/label="TK_PA_terminator"
/translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN
HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH
PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR
VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV
PAPDRTIASHRPCAQAASSKLPSTKL*"
CDS complement(1617..2642)
/label="ORF frame 1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene complement(1620..2627)
/label="hygroB"
/gene="hygroB"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
CDS 1884..3254
/label="ORF frame 3"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
promoter 2761..2909
/label="TATA_promoter"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
gene 2916..3254
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
CDS 3158..3769
/label="ORF frame 2"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
gene complement(4203..5063)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
CDS complement(4203..5063)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5105..5133)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 5804..6925
/label="ORF frame 2"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR
GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD
VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR
ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA
PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD
GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*"
rep_origin 7229..9399
/label="EBV_origin"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR
GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD
VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR
ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA
PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD
GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*"
ORIGIN
1 tctagagtcg accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg
61 gtgcactctc agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc
121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
241 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg
301 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
361 gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt
421 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
481 tggtacgatc gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc
541 actgaattcc gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc
601 catttgacca ttcaccacat tggtgtgcac ctccaagctg ggtaccagct gctagcaagc
661 ttgctagcgg ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg
721 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
781 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
841 gcattcattt tatgtttcag gttcaggggg aggtggggag gttttttaaa gcaagtaaaa
901 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
961 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
1021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
1081 gccatgaccg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
1141 gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
1201 aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca
1261 acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
1321 aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
1381 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
1441 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
1501 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
1561 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat
1621 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
1681 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
1741 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
1801 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
1861 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
1921 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
1981 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
2041 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
2101 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
2161 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
2221 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
2281 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
2341 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
2401 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
2461 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
2521 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
2581 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
2641 atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg
2701 gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc
2761 gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt
2821 gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac
2881 gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct
2941 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
3001 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
3061 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
3121 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
3181 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
3241 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
3301 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
3361 atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt
3421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
4021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
4081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
4141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
4201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
4261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
4321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
4381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
4441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
4501 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
4561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
4621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
4681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
4741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
4801 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
4861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
4921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
4981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
5041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
5161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
5221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
5281 ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
5341 ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
5401 catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
5461 acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
5521 acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
5581 aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
5641 tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
5701 gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
5761 ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
5821 agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
5881 agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
5941 acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
6001 gggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg gaggccgccg
6061 gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg
6121 tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc
6181 accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg aagccgatta
6241 ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc cgggagcgat
6301 agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc ggggtcaggg
6361 tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc aaggaggttc
6421 caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta ggagtcacgt
6481 agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa
6541 gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat gtcgtcttac
6601 accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac ctggcccgct
6661 aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat ttgctgaggt
6721 tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct gcaatatcag
6781 ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc cacctatggt
6841 ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag gtgatggaga
6901 tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca atcgtattaa
6961 aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg cgtgctgttg
7021 gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg ggcaattggg
7081 catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca
7141 ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa
7201 attcacctaa gaatgggagc aaccagcatg caggaaaagg acaagcagcg aaaattcacg
7261 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
7321 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
7381 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
7441 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
7501 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
7561 atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
7621 gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
7681 gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
7741 gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
7801 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
7861 gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
7921 cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
7981 cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
8041 gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
8101 tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
8161 acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
8221 tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
8281 agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
8341 ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
8401 ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
8461 atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
8521 atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
8581 gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
8641 attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
8701 gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
8761 attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
8821 gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
8881 gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
8941 ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
9001 ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
9061 ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
9121 cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
9181 ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
9241 gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
9301 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
9361 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gaatacaaaa
9421 caaaagcgct cctcgtacca gcgaagaagg ggcagagatg ccgtagtcag gtttagttcg
9481 tccggcggcg gggc
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