| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pEBVHisC |
| 质粒类型: | 哺乳动物表达载体；附加体载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | RSV |
| 载体大小: | 10029 bp |
| 5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | His tag (N-term) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | DH5α等 |
| 宿主细胞（系）: | 人类细胞；灵长类细胞；犬类细胞 |
| 备注: | pEBVHisC载体是附加体型载体，可以在基因组外自主复制，不需要整合和转座到基因组上，无需包装成病毒，即可实现稳定表达。其它附加体载体有： pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
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- 已注册
pEBVHisC
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEBVHisC 10315 bp DNA SYN
DEFINITION pEBVHisC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10315
/organism="pEBVHisC"
/mol_type="other DNA"
misc_feature 112..134
/label="pGEX_3_primer"
terminator 279..410
/label="SV40_PA_terminator"
misc_feature 367..386
/label="EBV_rev_primer"
misc_feature complement(506..529)
/label="Xpress_EK"
misc_feature complement(533..565)
/label="T7_leader"
misc_feature complement(548..566)
/label="Xpress_fwd_primer"
misc_feature complement(632..650)
/label="pREP_fwd_primer"
CDS 1211..1948
/label="ORF frame 2"
/translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
VPAPDRTIASHRPCAQAASSKLPSTKL*"
terminator complement(1469..1739)
/label="TK_PA_terminator"
/translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
VPAPDRTIASHRPCAQAASSKLPSTKL*"
CDS complement(1747..2772)
/label="ORF frame 2"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene complement(1750..2757)
/label="hygroB"
/gene="hygroB"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
CDS 2014..3384
/label="ORF frame 1"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
promoter 2891..3039
/label="TATA_promoter"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
gene 3046..3384
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
CDS 3288..3899
/label="ORF frame 3"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
gene complement(4333..5193)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
CDS complement(4333..5193)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5235..5263)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS complement(5819..7033)
/label="ORF frame 1"
/translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW
LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL
PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL
PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL
LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP
LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS
GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG
HFQVLYLAPRQT*"
CDS 5821..7746
/label="ORF frame 1"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
DDGDEGGDGDEGEEGQE*"
CDS 5936..7048
/label="ORF frame 2"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
rep_origin 8050..10220
/label="EBV_origin"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
481 gatctcgagg tcggatccat cgatccttat cgtcatcgtc gtacagatcc cgacccattt
541 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa ccccccatgg
601 aggtgcacac caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta
661 aatacaatta tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc
721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
841 aagtgtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
901 tgaggggact cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt
961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
1141 cggcagcaga gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa
1201 gctgtcaaac atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg
1261 ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg
1321 gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag
1381 accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga
1441 ccccgtccct aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct
1501 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg
1561 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
1621 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
1681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
1741 tctcccctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
1801 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
1861 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
1921 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
1981 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
2041 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
2101 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
2161 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
2221 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
2281 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
2341 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
2401 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
2461 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
2521 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
2581 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
2641 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
2701 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
2761 aggctttttc atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg
2821 tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac
2881 ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg
2941 ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc
3001 gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg
3061 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
3121 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
3181 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
3241 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
3301 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
3361 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
3421 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
3481 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc
3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
4261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
4321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
4381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
4501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
4561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
4621 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
4681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
4741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
4801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
4861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
4921 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
4981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
5221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
5281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
5341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
5401 attctcatgt ttgacagctt atcatcgcga taagctgatc ctcacaggcc gcacccagct
5461 tttcttccgt tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt
5521 cccgagaatc cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc
5581 ttgaggctca ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa
5641 ttggtgatga ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg
5701 acagcgacca tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact
5761 ccctgtattc actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc
5821 atgtctgacg aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca
5881 tctggaccag aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga
5941 cgaggacggg gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca
6001 ggatcagggc caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt
6061 ggctgcaaag ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca
6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
6181 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
6241 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
6301 ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg
6361 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
6421 ggggcaggag caggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca
6481 ggaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggggca
6541 ggagcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg
6601 gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
6661 ggaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
6721 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
6781 ggggcaggag caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga
6841 ggtagtggag gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga
6901 gccagggggg gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg
6961 cccaggagtc ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca
7021 ggtagaaggc catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa
7081 ggtggcccag atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat
7141 gacccaggag aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa
7201 aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac
7261 attgcagaag gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa
7321 ggaacttggg tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta
7381 aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc
7441 tttggaatgg cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt
7501 tatttcatgg tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag
7561 gaccttgtta tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt
7621 gacgatggag tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag
7681 ggtgatgacg gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag
7741 gagtgatgta acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc
7801 actaaagaat aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg
7861 tcttgtcacc attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt
7921 cactcagctc cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt
7981 gagcaatcag acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag
8041 caaccagcat gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg
8101 catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc
8161 atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
8221 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
8281 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
8341 attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag
8401 attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta
8461 ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta
8521 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
8581 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
8641 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
8701 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
8761 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
8821 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
8881 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
8941 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
9001 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
9061 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
9121 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
9181 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
9241 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
9301 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
9361 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
9421 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
9481 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
9541 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
9601 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
9661 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
9721 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
9781 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
9841 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
9901 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
9961 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
10021 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
10081 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
10141 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
10201 aaccaactga agggattaca tgcactgccc cgaatacaaa acaaaagcgc tcctcgtacc
10261 agcgaagaag gggcagagat gccgtagtca ggtttagttc gtccggcggc ggggc
//
LOCUS pEBVHisC 10315 bp DNA SYN
DEFINITION pEBVHisC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10315
/organism="pEBVHisC"
/mol_type="other DNA"
misc_feature 112..134
/label="pGEX_3_primer"
terminator 279..410
/label="SV40_PA_terminator"
misc_feature 367..386
/label="EBV_rev_primer"
misc_feature complement(506..529)
/label="Xpress_EK"
misc_feature complement(533..565)
/label="T7_leader"
misc_feature complement(548..566)
/label="Xpress_fwd_primer"
misc_feature complement(632..650)
/label="pREP_fwd_primer"
CDS 1211..1948
/label="ORF frame 2"
/translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
VPAPDRTIASHRPCAQAASSKLPSTKL*"
terminator complement(1469..1739)
/label="TK_PA_terminator"
/translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
VPAPDRTIASHRPCAQAASSKLPSTKL*"
CDS complement(1747..2772)
/label="ORF frame 2"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene complement(1750..2757)
/label="hygroB"
/gene="hygroB"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
CDS 2014..3384
/label="ORF frame 1"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
promoter 2891..3039
/label="TATA_promoter"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
gene 3046..3384
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
CDS 3288..3899
/label="ORF frame 3"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
gene complement(4333..5193)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
CDS complement(4333..5193)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5235..5263)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS complement(5819..7033)
/label="ORF frame 1"
/translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW
LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL
PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL
PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL
LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP
LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS
GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG
HFQVLYLAPRQT*"
CDS 5821..7746
/label="ORF frame 1"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
DDGDEGGDGDEGEEGQE*"
CDS 5936..7048
/label="ORF frame 2"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
rep_origin 8050..10220
/label="EBV_origin"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
481 gatctcgagg tcggatccat cgatccttat cgtcatcgtc gtacagatcc cgacccattt
541 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa ccccccatgg
601 aggtgcacac caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta
661 aatacaatta tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc
721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
841 aagtgtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
901 tgaggggact cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt
961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
1141 cggcagcaga gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa
1201 gctgtcaaac atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg
1261 ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg
1321 gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag
1381 accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga
1441 ccccgtccct aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct
1501 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg
1561 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
1621 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
1681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
1741 tctcccctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
1801 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
1861 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
1921 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
1981 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
2041 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
2101 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
2161 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
2221 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
2281 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
2341 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
2401 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
2461 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
2521 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
2581 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
2641 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
2701 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
2761 aggctttttc atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg
2821 tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac
2881 ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg
2941 ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc
3001 gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg
3061 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
3121 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
3181 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
3241 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
3301 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
3361 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
3421 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
3481 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc
3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
4261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
4321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
4381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
4501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
4561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
4621 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
4681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
4741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
4801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
4861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
4921 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
4981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
5221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
5281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
5341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
5401 attctcatgt ttgacagctt atcatcgcga taagctgatc ctcacaggcc gcacccagct
5461 tttcttccgt tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt
5521 cccgagaatc cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc
5581 ttgaggctca ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa
5641 ttggtgatga ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg
5701 acagcgacca tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact
5761 ccctgtattc actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc
5821 atgtctgacg aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca
5881 tctggaccag aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga
5941 cgaggacggg gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca
6001 ggatcagggc caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt
6061 ggctgcaaag ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca
6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
6181 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
6241 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
6301 ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg
6361 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
6421 ggggcaggag caggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca
6481 ggaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggggca
6541 ggagcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg
6601 gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
6661 ggaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
6721 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
6781 ggggcaggag caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga
6841 ggtagtggag gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga
6901 gccagggggg gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg
6961 cccaggagtc ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca
7021 ggtagaaggc catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa
7081 ggtggcccag atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat
7141 gacccaggag aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa
7201 aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac
7261 attgcagaag gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa
7321 ggaacttggg tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta
7381 aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc
7441 tttggaatgg cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt
7501 tatttcatgg tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag
7561 gaccttgtta tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt
7621 gacgatggag tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag
7681 ggtgatgacg gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag
7741 gagtgatgta acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc
7801 actaaagaat aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg
7861 tcttgtcacc attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt
7921 cactcagctc cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt
7981 gagcaatcag acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag
8041 caaccagcat gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg
8101 catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc
8161 atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
8221 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
8281 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
8341 attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag
8401 attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta
8461 ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta
8521 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
8581 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
8641 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
8701 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
8761 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
8821 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
8881 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
8941 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
9001 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
9061 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
9121 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
9181 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
9241 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
9301 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
9361 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
9421 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
9481 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
9541 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
9601 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
9661 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
9721 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
9781 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
9841 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
9901 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
9961 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
10021 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
10081 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
10141 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
10201 aaccaactga agggattaca tgcactgccc cgaatacaaa acaaaagcgc tcctcgtacc
10261 agcgaagaag gggcagagat gccgtagtca ggtttagttc gtccggcggc ggggc
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