BioVector® NPC43 人鼻咽癌细胞株 / NPC43 Human Nasopharyngeal Carcinoma Cell Line

一、 背景与来源说明 / Background & Origin

NPC43 是一种来源于人鼻咽癌（Nasopharyngeal Carcinoma, NPC）患者原发肿瘤组织的永生化肿瘤细胞株。由于大多数常见鼻咽癌细胞株在体外长期传代过程中容易丢失 EB 病毒（Epstein-Barr Virus, EBV），NPC43 是少数能够稳定携带并维持 EB 病毒（EBV-positive）的患者来源鼻咽癌细胞模型之一。NPC43 细胞株广泛应用于 EBV 阳性鼻咽癌发病机制、EB 病毒潜伏感染与致癌基因表达、肿瘤免疫微环境、放化疗敏感性及靶向药物筛选的研究。

二、 细胞生物学特征 / Biological Characteristics

• 组织来源 / Tissue Origin：人鼻咽癌原发肿瘤组织 (Human Nasopharyngeal Carcinoma)

• 生长特性 / Growth Properties：贴壁生长，呈多角形或上皮样形态

• 关键病毒特征 / EBV Status：EB 病毒阳性 (EBV-Positive)

• 细胞标记物 / Cell Markers：

  • 上皮细胞标志物：细胞角蛋白 (Cytokeratin, CK)

  • EBV 基因表达产品：EBNA1, LMP1, EBERs

• 应用领域 / Applications：EBV 相关鼻咽癌病理机制研究、EBV 潜伏与激活调控、抗 EBV 靶向疗法评估及放射治疗增敏药物筛选

三、 培养条件与传代指南 / Culture & Handling Protocols

1. 完全培养基配方 / Medium Formulation

• 基础培养基 / Basal Medium：RPMI-1640 基础培养基

• 血清与添加剂 / Serum & Supplements：

  • 10% 至 15% 胎牛血清 (FBS)

  • ROCK 抑制剂（如 Y-27632，4 至 10 uM，依建系与消化复苏需要添加）

• 抗生素 / Antibiotics：100 U/mL 青霉素 + 100 ug/mL 链霉素

2. 培养与传代操作 / Incubation & Subculture

• 培养环境 / Conditions：37 摄氏度，5% CO2，饱和湿度

• 密度管理 / Confluence：保持细胞密度在 30% 到 85% 之间，避免过度汇合导致 EB 病毒脱落或细胞自发凋亡

• 传代步骤 / Procedure：

  1. 吸除旧培养基，用 PBS 洗涤一次。

  2. 加入 0.25% Trypsin-EDTA，在 37 摄氏度下孵育 2 到 4 分钟至细胞脱落。

  3. 加入含血清完全培养基终止消化并轻柔吹打均匀。

  4. 以 200 x g 离心 5 分钟，重悬后按 1:2 至 1:3 比例接种。

四、 技术指标简表 / Technical Data Summary

BioVector® NPC43 Human Nasopharyngeal Carcinoma Cell Line

1. Background and Origin

NPC43 is a patient-derived cell line established from human nasopharyngeal carcinoma (NPC) tissue. Unlike many classical NPC lines that tend to lose the Epstein-Barr virus (EBV) during prolonged in vitro passage, NPC43 is one of the rare cell models that stably retains the EBV genome (EBV-positive). NPC43 serves as a vital model for investigating EBV-driven nasopharyngeal oncogenesis, viral latency/reactivation, tumor immunology, and therapeutic drug screening.

2. Biological Characteristics

• Tissue Origin: Human Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent, epithelial-like / polygonal morphology

• EBV Status: EBV-positive

• Cell Markers:

  • Epithelial markers: Cytokeratin (CK)

  • EBV-encoded gene products: EBNA1, LMP1, EBERs

• Applications: EBV-associated NPC pathogenesis, EBV latency regulation, immunotherapeutic targeting, and radiosensitization drug discovery

3. Culture and Handling Protocols

Culture Medium Formulation

• Basal Medium: RPMI-1640

• Serum and Additives: 10 to 15 percent Fetal Bovine Serum (FBS), supplemented with ROCK inhibitor (Y-27632, 4 to 10 uM) as required for lineage maintenance

• Antibiotics: 100 U/mL Penicillin and 100 ug/mL Streptomycin

Incubation and Subculture Guide

• Incubation Conditions: 37 degrees Celsius, 5 percent CO2, humidified atmosphere

• Confluence Management: Maintain cell density between 30 percent and 85 percent to prevent EBV loss or cell detachment

• Subculture Procedure:

  1. Remove spent media and wash once with PBS.

  2. Add 0.25 percent Trypsin-EDTA and incubate at 37 degrees Celsius for 2 to 4 minutes until cells detach.

  3. Neutralize with complete medium and mix gently.

  4. Centrifuge at 200 x g for 5 minutes, resuspend, and seed at a ratio between 1:2 and 1:3.

4. Technical Data Summary

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