C6-36-HE8-Act::Cas9-2A-Neo细胞株NTCC® Cell Line BioVector细胞保藏中心

[Designation名称] NTCC® C6-36-HE8-Act::Cas9-2A-Neo Cell Line细胞株
BioVector NTCC典型培养物保藏中心

ID C6/36-HE8-Act::Cas9-2A-Neo
Cross-references: DGRC; 363
Cross-references: FlyBase_Cell_line; FBtc0000363
References identifiers: PubMed=34819517;
Comments: Group: Insect cell line.
Comments: Characteristics: Modified using a Minos mediated integration cassette (MiMIC) to deliver a recombination-mediated cassette (RMCE) acceptor, to add a docking site for recombination via the bacteriophage FC31 integrase.
Comments: Characteristics: A N-3xFLAG-spCas9-T2A-Neo construct was integrated in the Act5C locus (PubMed=34819517).
Comments: Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Species of origin: NCBI_TaxID=7160; ! Aedes albopictus (Asian tiger mosquito)
Hierarchy: CVCL_B3N5 ! C6/36-HE8
[Organism物种来源]
[Disease疾病种类]
[编号]Accession Number: NTCC® CVCL_F1ZQ
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® C6-36-HE8-Act::Cas9-2A-Neo Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

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