BioVector® HCCLM9 人高转移肝癌细胞系 / BioVector® HCCLM9 Human High-Metastatic Hepatocellular Carcinoma Cell Line

通用定义 / General Definition:

BioVector® HCCLM9 是一种具有极高转移潜能的人源肝细胞癌（HCC）细胞系。该细胞系是通过将人肝癌细胞在裸鼠体内进行多次原位接种和肺转移筛选而建立的“LM”系列（Liver Metastasis）中的高端型号。与较低转移能力的 HCCLM3 相比，HCCLM9 表现出更强的上皮-间充质转化（EMT）特征、更高的侵袭力以及极高的肺转移率。它是研究肝癌转移分子机制、肿瘤血管生成以及评价抗转移药物疗效的理想模型。

BioVector® HCCLM9 is a human highly metastatic hepatocellular carcinoma (HCC) cell line. It was established as part of the "LM" (Liver Metastasis) series through successive rounds of orthotopic implantation and pulmonary metastasis screening in nude mice. Compared to the lower-metastatic HCCLM3, HCCLM9 exhibits enhanced epithelial-mesenchymal transition (EMT) features, greater invasiveness, and a significantly higher rate of spontaneous lung metastasis. It serves as a premier model for investigating the molecular mechanisms of HCC metastasis, tumor angiogenesis, and evaluating the efficacy of anti-metastatic therapeutic agents.

BioVector® HCCLM9 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® HCCLM9 人高转移肝癌细胞

• 组织来源： 肝脏 (Liver)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样，呈多角形或纺锤形，生长密集时呈铺路石状。

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基（高糖）。

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:3 至 1:6；该细胞生长迅速，建议在汇合度达到 80-90% 时及时传代。

3. 细胞应用

• 转移机制研究： 研究循环肿瘤细胞（CTC）的形成及远处器官（如肺部）定殖的生物学过程。

• EMT 转化分析： 探索 Snail, Twist, Vimentin 等标志物在肝癌细胞获得侵袭能力中的作用。

• 动物造模： 用于构建裸鼠肝癌原位移植模型或肺转移模型，观察药物对肿瘤生长和转移的抑制。

4. 注意事项

• 遗传稳定性： 随着传代次数增加，细胞的高转移特性可能出现减弱。BioVector® 建议实验使用复苏后 10 代以内的细胞。

• 细胞接种： 进行体内转移实验时，需严格控制接种细胞的数量和活力，以确保肺转移结节的可重复性。

English Datasheet

1. General Product Information

• Product Name: BioVector® HCCLM9 Human Highly Metastatic Hepatocellular Carcinoma Cell Line

• Tissue Source: Liver

• Growth Properties: Adherent

• Morphology: Epithelial-like; polygonal or spindle-shaped, forming a cobblestone pattern at high density.

2. Culture Conditions

• Basal Medium: BioVector® DMEM (High Glucose).

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:3 to 1:6 ratio; due to its rapid proliferation, subculture when cells reach 80-90% confluence.

3. Applications

• Metastasis Research: Studying the biological stages of the metastatic cascade, from intravasation to successful colonization in distant organs like the lungs.

• EMT Analysis: Investigating the signaling pathways and transcription factors (e.g., TGF-$\beta$, Snail) that drive the conversion from a sedentary to a migratory phenotype.

• In Vivo Modeling: Creating orthotopic or tail-vein injection models in nude mice to assess tumor burden and metastatic index.

4. Key Usage Notes

• Maintenance of Phenotype: The high metastatic potential of HCCLM9 can drift over long-term culture. BioVector® recommends using low-passage cells (within 10 passages post-thawing) for critical metastasis assays.

• Culture Quality: Ensure the use of high-glucose DMEM to support the aggressive metabolic demands of this highly malignant cell line.

注意 / Note: BioVector® HCCLM9 应在生物安全二级 (BSL-2) 条件下操作。由于其极强的侵袭性和增殖能力，它是开发靶向肝癌转移药物（如 VEGF 或 MET 抑制剂）的关键工具。

BioVector® HCCLM9 should be handled under Biosafety Level 2 (BSL-2) conditions. Given its aggressive invasive and proliferative capacity, it is a pivotal tool for developing drugs targeting HCC metastasis, such as VEGF or MET inhibitors.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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