BioVector® MH-S 小鼠肺泡巨噬细胞系 / BioVector® MH-S Mouse Alveolar Macrophage Cell Line

通用定义 / General Definition:

BioVector® MH-S 是一种源自 BALB/c 小鼠的永生化肺泡巨噬细胞系。该细胞系通过 SV40 病毒转化建立，保留了肺泡巨噬细胞的关键生理特征。MH-S 细胞具有活跃的吞噬能力，能够产生细胞因子，并表达巨噬细胞特异性表面标志物。作为研究肺部免疫学、呼吸道感染、哮喘、肺纤维化以及环境颗粒物（如 PM2.5）毒理学的标准模型，MH-S 提供了比原代细胞更高的实验重现性和操作便捷性。

BioVector® MH-S is an immortalized alveolar macrophage cell line derived from BALB/c mice. Established via SV40 transformation, the cell line retains the essential physiological characteristics of primary alveolar macrophages. MH-S cells exhibit active phagocytic capacity, produce various cytokines, and express macrophage-specific surface markers. As a standard model for studying pulmonary immunology, respiratory infections, asthma, lung fibrosis, and the toxicology of environmental particulates (e.g., PM2.5), MH-S offers superior experimental reproducibility and ease of handling compared to primary cells.

BioVector® MH-S 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® MH-S 小鼠肺泡巨噬细胞

• 组织来源： 肺 (Lung/Alveoli)

• 生长特性： 贴壁生长（有时伴有少量悬浮细胞）

• 细胞形态： 巨噬细胞样，呈圆形或多角形，具有典型的伪足结构。

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 培养基。

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)。

• 关键添加剂： 需添加 0.05 mM $\beta$-巯基乙醇 ($\beta$-mercaptoethanol) 以维持细胞活性。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:2 至 1:4；建议使用细胞刮刀（Scraper）或轻微胰酶消化进行传代，避免过度消化损伤膜受体。

3. 细胞应用

• 呼吸道感染研究： 研究肺部病原体（如结核杆菌、流感病毒）与巨噬细胞的相互作用。

• 炎症反应： 评估 LPS 或其他刺激物诱导的促炎细胞因子（TNF-$\alpha$, IL-6）释放。

• 吞噬实验： 定量检测细胞对荧光标记细菌或环境尘埃颗粒的吞噬效率。

4. 注意事项

• 贴壁性： MH-S 细胞贴壁较牢固，但在生长过快或营养不足时可能出现部分脱落悬浮，这属于正常现象。

• 激活状态： 巨噬细胞对培养基中的内毒素非常敏感，BioVector® 建议使用极低内毒素含量的 FBS。

English Datasheet

1. General Product Information

• Product Name: BioVector® MH-S Mouse Alveolar Macrophage Cell Line

• Tissue Source: Lung (Alveoli)

• Growth Properties: Adherent (occasionally with some cells in suspension)

• Morphology: Macrophage-like; round or polygonal with characteristic pseudopodia.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 Medium.

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Key Supplements: Supplemented with 0.05 mM $\beta$-mercaptoethanol to maintain cellular redox balance and viability.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:2 to 1:4 ratio; use a cell scraper or gentle trypsinization. Avoid prolonged enzymatic treatment to preserve membrane-bound receptors.

3. Applications

• Respiratory Infection Modeling: Investigating the interaction between pulmonary pathogens (e.g., M. tuberculosis, Influenza virus) and host immune cells.

• Inflammatory Response: Measuring the release of pro-inflammatory cytokines (TNF-$\alpha$, IL-6) induced by LPS or other irritants.

• Phagocytosis Assays: Quantifying the uptake efficiency of fluorescently labeled bacteria or environmental particulate matter.


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4. Key Usage Notes

• Adhesion Profile: Healthy MH-S cells form a stable monolayer, but partial detachment may occur during rapid proliferation or nutrient depletion.

• Activation Sensitivity: Macrophages are highly reactive to endotoxins. BioVector® recommends the use of ultra-low endotoxin FBS and certified pyrogen-free plasticware to prevent baseline activation.

注意 / Note: BioVector® MH-S 应在生物安全二级 (BSL-2) 条件下操作。该细胞系是替代原代支气管肺泡灌洗 (BAL) 细胞的高效工具，非常适合高通量筛选实验。

BioVector® MH-S should be handled under Biosafety Level 2 (BSL-2). This cell line is a highly efficient alternative to primary Bronchoalveolar Lavage (BAL) cells and is particularly well-suited for high-throughput screening assays.

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