BioVector® PLB-985 人急性髓系白血病细胞系 / BioVector® PLB-985 Human Acute Myeloid Leukemia Cell Line

通用定义 / General Definition:

BioVector® PLB-985 是一种源自人类急性髓系白血病 (AML) 的骨髓样细胞系。尽管最初被认为是一个独立的细胞系，但后来的遗传鉴定显示它与 HL-60 细胞系高度相似。PLB-985 的核心价值在于其强大的粒细胞/单核细胞分化潜能。通过二甲基亚砜 (DMSO) 或全反式维甲酸 (ATRA) 的诱导，PLB-985 可以高度模拟中性粒细胞的生理功能，特别是产生活性氧 (ROS) 的能力。它是研究中性粒细胞功能、NADPH 氧化酶复合体机制以及炎症反应的理想模型，常被用于开发针对慢性肉芽肿病 (CGD) 的基因疗法。

BioVector® PLB-985 is a myeloid cell line derived from human Acute Myeloid Leukemia (AML). Although initially described as a distinct line, subsequent genetic profiling revealed it to be a subline of HL-60. The primary value of PLB-985 lies in its robust potential for granulocytic and monocytic differentiation. Upon induction with agents like DMSO or All-Trans Retinoic Acid (ATRA), PLB-985 cells effectively mimic the physiological functions of mature neutrophils, particularly the ability to generate Reactive Oxygen Species (ROS). It is an ideal model for studying neutrophil biology, the NADPH oxidase complex, and inflammatory responses, and it is frequently used in the development of gene therapies for Chronic Granulomatous Disease (CGD).

BioVector® PLB-985 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® PLB-985 人髓系白血病细胞

• 组织来源： 骨髓 (Bone Marrow)

• 生长特性： 悬浮生长 (Suspension)

• 细胞形态： 原始髓系细胞样，呈圆形或卵圆形，分化后体积缩小且核浆比改变。

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 培养基。

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 维护密度： 保持细胞密度在 $2 \times 10^5$ 至 $1 \times 10^6$ cells/mL 之间。

3. 诱导分化方案

• 中性粒细胞样分化： 使用 1.25% DMSO 或 1 $\mu$M ATRA 处理 4-6 天。

• 功能鉴定： 诱导后可通过 NBT 还原实验或细胞色素 C 还原法检测超氧化物产量。

4. 细胞应用

• NADPH 氧化酶研究： 利用 CRISPR/Cas9 敲除特定亚基（如 gp91phox）来模拟 CGD 病理。

• 趋化性分析： 研究分化后的 PLB-985 细胞对趋化因子（如 fMLP）的定向迁移。

• 吞噬作用： 评估细胞对细菌或荧光颗粒的吞噬效率。

5. 注意事项

• 分化效率： 细胞的代数会显著影响分化效率，建议使用复苏后 15 代以内的细胞进行诱导。

• 结团现象： 细胞在高密度时可能出现轻微结团，传代时应轻轻吹打分散。

English Datasheet

1. General Product Information

• Product Name: BioVector® PLB-985 Human Myeloid Leukemia Cell Line

• Tissue Source: Bone Marrow

• Growth Properties: Suspension

• Morphology: Myeloblast-like; round or oval cells that decrease in size and show nuclear remodeling upon differentiation.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 Medium.

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Maintenance: Maintain cell density between $2 \times 10^5$ and $1 \times 10^6$ cells/mL.

3. Differentiation Protocol

• Neutrophilic Differentiation: Treat with 1.25% DMSO or 1 $\mu$M ATRA for 4–6 days.

• Functional Validation: Post-differentiation, superoxide production can be measured via Nitroblue Tetrazolium (NBT) reduction or cytochrome c reduction assays.

4. Applications

• NADPH Oxidase Studies: Using CRISPR/Cas9 to knock out specific subunits (e.g., gp91phox) to model Chronic Granulomatous Disease (CGD).

• Chemotaxis Assays: Investigating the directional migration of differentiated PLB-985 cells toward chemoattractants like fMLP.

• Phagocytosis: Evaluating the efficiency of the cells in engulfing bacteria or fluorescently labeled beads.

5. Key Usage Notes

• Passage Effect: Differentiation efficiency declines with increasing passage number; BioVector® recommends using cells within 15 passages post-thawing for induction experiments.

• Cell Density: Overgrowth can lead to spontaneous differentiation or loss of potency; ensure regular subculturing to maintain the undifferentiated precursor state.

注意 / Note: BioVector® PLB-985 应在生物安全二级 (BSL-2) 条件下操作。该细胞系是研究先天免疫防御机制的高效替代模型，尤其适用于需要大量中性粒细胞样细胞的实验。

BioVector® PLB-985 should be handled under Biosafety Level 2 (BSL-2). This cell line serves as a high-efficiency alternative model for studying innate immune defense mechanisms, particularly in experiments requiring large quantities of neutrophil-like cells.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail: BioVector@163.com

http://www.biovector.net