BioVector® VM7luc4E2 人乳腺癌雌激素受体报告基因细胞系 / BioVector® VM7luc4E2 Human Breast Cancer ER Reporter Cell Line

通用定义 / General Definition:

BioVector® VM7luc4E2（又名 VM7 或经过改进的 BG1Luc4E2）是一种高度敏感的人源报告基因细胞系，专门用于检测内分泌干扰物 (EDCs) 和雌激素活性。该细胞系由人乳腺腺癌细胞衍生而来，稳定转染了由雌激素应答元件 (ERE) 驱动的萤火虫荧光素酶 (Firefly Luciferase) 报告基因质粒。由于其内源性表达高水平的人雌激素受体（$\alpha$ 和 $\beta$），VM7luc4E2 被美国环保署 (EPA) 和 OECD 认可为评估化学品雌激素受体激动或拮抗活性的标准体外实验模型。

BioVector® VM7luc4E2 (also known as VM7 or optimized BG1Luc4E2) is a highly sensitive human-derived reporter cell line specifically designed for the detection of Endocrine Disrupting Chemicals (EDCs) and estrogenic activity. Derived from a human breast adenocarcinoma lineage, these cells are stably transfected with a Firefly Luciferase reporter gene plasmid driven by Estrogen Response Elements (ERE). Expressing high levels of endogenous human estrogen receptors ($\alpha$ and $\beta$), VM7luc4E2 is recognized by the US EPA and OECD as a gold-standard in vitro model for assessing the estrogen receptor agonistic or antagonistic activities of chemicals.

BioVector® VM7luc4E2 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® VM7luc4E2 雌激素报告基因细胞系

• 母本细胞： VM7 (原名 BG-1, 人乳腺癌细胞)

• 生长特性： 贴壁生长

• 报告基因： ERE-驱动的萤火虫荧光素酶 (Firefly Luciferase)

• 细胞形态： 上皮样，呈多角形

2. 培养条件

• 维持培养基： BioVector® DMEM/F12 (无酚红) 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 筛选抗生素： 含有适量的 G418 以维持报告基因的稳定性。

• 实验前处理： 为降低背景活性，实验前通常需在含有 5% 活性炭脱色血清 (DCC-FBS) 的无酚红培养基中饥饿处理 24-48 小时。

• 培养环境： 37 摄氏度，5% CO2

3. 细胞应用

• 内分泌干扰筛选： 定量检测农药、化妆品成分或工业化学品的类雌激素活性。

• 药物开发： 评估新型选择性雌激素受体调节剂 (SERMs) 的效能。

• 环境监测： 监测水质或土壤提取物中的生物有效性雌激素浓度。

4. 注意事项

• 酚红敏感性： 酚红具有微弱的雌激素活性，所有功能性实验必须使用无酚红培养基。

• 血清选择： 必须使用经过活性炭处理的血清 (DCC-FBS) 以去除内源性类固醇激素，否则会导致背景信号过高。

English Datasheet

1. General Product Information

• Product Name: BioVector® VM7luc4E2 Human Breast Cancer ER Reporter Cell Line

• Parental Line: VM7 (formerly BG-1, Human Breast Adenocarcinoma)

• Growth Properties: Adherent

• Reporter Gene: ERE-driven Firefly Luciferase

• Morphology: Epithelial-like, polygonal.

2. Culture Conditions

• Maintenance Medium: BioVector® DMEM/F12 (Phenol Red-Free) Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Selection Marker: Maintain with G418 to ensure the stability of the luciferase expression cassette.

• Assay Preparation: To minimize background signaling, cells are typically starved in medium containing 5% Dextran-Coated Charcoal-stripped FBS (DCC-FBS) for 24-48 hours prior to chemical exposure.

• Incubation: 37 degrees Celsius, 5% CO2.

3. Applications

• Endocrine Disruptor Screening: Quantifying the estrogenic potential of pesticides, cosmetic ingredients, or industrial chemicals (e.g., BPA).

• Pharmaceutical Research: Evaluating the potency of novel Selective Estrogen Receptor Modulators (SERMs).

• Environmental Monitoring: Detecting bioavailable estrogenic activity in water samples or environmental leachates.

4. Key Usage Notes

• Phenol Red Interference: Phenol red acts as a weak estrogen mimic; therefore, Phenol Red-Free media must be used for all transactivation assays.

• Hormone Depletion: The use of DCC-FBS is critical during the assay phase to remove endogenous hormones that would otherwise cause high baseline luciferase activity.

注意 / Note: BioVector® VM7luc4E2 具有极高的灵敏度，操作过程中应严格防止实验室塑料制品的潜在浸出物（如邻苯二甲酸酯）干扰实验。

BioVector® VM7luc4E2 is extremely sensitive; precautions should be taken to prevent interference from potential leachable endocrine disruptors (e.g., phthalates) found in laboratory plasticware.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail: BioVector@163.com

http://www.biovector.net