BioVector® RPMI-8866 人 B 淋巴母细胞系 / BioVector® RPMI-8866 Human B Lymphoblastoid Cell Line

通用定义 / General Definition:BioVector® RPMI-8866 是一种源自人类外周血的内源性 EB 病毒 (EBV) 转化的 B 淋巴母细胞系。该细胞系最初建立于 20 世纪 60 年代（来自一名患有慢性髓细胞白血病的患者），但由于其成熟的 B 细胞特征，它被广泛用作免疫学研究的标准模型。RPMI-8866 细胞稳定表达多种细胞表面标志物，包括 CD23 和 MHC II 类分子，使其成为研究 B 细胞激活、抗原呈递、免疫球蛋白合成以及细胞因子调控的理想工具。

BioVector® RPMI-8866 is a human B lymphoblastoid cell line derived from peripheral blood and transformed by endogenous Epstein-Barr Virus (EBV). Originally established in the 1960s (from a patient with chronic myelogenous leukemia), it has since become a standard model in immunology due to its mature B-cell characteristics. RPMI-8866 cells constitutively express various surface markers, including CD23 and MHC class II molecules, making them an ideal tool for studying B-cell activation, antigen presentation, immunoglobulin synthesis, and cytokine regulation.

BioVector® RPMI-8866 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® RPMI-8866 人 B 淋巴母细胞

• 组织来源： 外周血 (Peripheral Blood)

• 生长特性： 悬浮生长 (Suspension)

• 细胞形态： 淋巴母细胞样，通常呈圆形，单个或成簇悬浮。

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 培养基

• 血清添加： 10% - 15% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2

• 传代与维护： 细胞密度应维持在 $2 \times 10^5$ 至 $1 \times 10^6$ cells/mL 之间。传代时只需通过离心或稀释方式补充新鲜培养基，无需消化处理。

3. 细胞应用

• 免疫学研究： 研究 CD23（低亲和力 IgE 受体）的表达及其在过敏反应中的作用。

• 细胞因子分析： 评估 IL-4、IL-13 等细胞因子对 B 细胞功能的调节。

• EBV 病毒研究： 作为 EB 病毒永生化 B 细胞的模型，研究病毒潜伏感染机制。

4. 注意事项

• 结团现象： RPMI-8866 细胞在生长良好时倾向于形成明显的细胞团簇，这属于正常生理状态，通常通过轻轻吹打即可分散。

• 安全性： 由于该细胞系含有内源性 EB 病毒，必须在生物安全二级 (BSL-2) 环境下操作。

English Datasheet

1. General Product Information

• Product Name: BioVector® RPMI-8866 Human B Lymphoblastoid Cell Line

• Tissue Source: Peripheral Blood

• Growth Properties: Suspension

• Morphology: Lymphoblast-like; typically round cells growing individually or in clusters.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 Medium

• Serum Supplement: 10% - 15% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2

• Maintenance: Maintain cell density between $2 \times 10^5$ and $1 \times 10^6$ cells/mL. Subculture by dilution or centrifugation with fresh medium; no trypsinization is required for suspension cells.

3. Applications

• Immunology Research: Studying the expression of CD23 (the low-affinity IgE receptor) and its role in allergic responses.

• Cytokine Signaling: Evaluating the modulation of B-cell functions by cytokines such as IL-4 and IL-13.

• EBV Pathogenesis: Serving as a model for EBV-immortalized B cells to investigate viral latency and oncogenesis.

4. Key Usage Notes

• Cell Clumping: It is characteristic for healthy RPMI-8866 cells to form clusters in suspension. These aggregates can be gently dissociated by pipetting during subculturing.

• Safety Protocols: As the cell line harbors endogenous Epstein-Barr Virus, it must be handled under Biosafety Level 2 (BSL-2) guidelines.

注意 / Note: BioVector® 建议定期检查培养物的细胞密度，避免过度汇合导致营养耗竭。为确保实验的科学性，建议定期进行支原体检测。

BioVector® recommends regular monitoring of cell density to prevent nutrient depletion from overgrowth. Periodic mycoplasma screening is highly advised to ensure the integrity of your research.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail: BioVector@163.com

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