BioVector® MDCK-SIAT1 犬肾上皮细胞系 / BioVector® MDCK-SIAT1 Canine Kidney Epithelial Cell Line

通用定义 / General Definition:

BioVector® MDCK-SIAT1 是一种经过稳定转染的人源 SIAT1 (ST6Gal-I, $\alpha$2,6-唾液酸转移酶) 基因的 MDCK（Madin-Darby 犬肾细胞） 衍生细胞系。该细胞系的设计初衷是为了解决普通 MDCK 细胞在流感病毒研究中的局限性。人流感病毒优先结合 $\alpha$2,6-连接的唾液酸受体，而普通 MDCK 细胞表面主要表达 $\alpha$2,3-连接受体。通过过表达 SIAT1，MDCK-SIAT1 细胞能够模拟人类上呼吸道上皮的受体分布，从而显著提高人流感病毒的分离率和滴度，是流感病毒监测和疫苗研究的行业标准模型。

BioVector® MDCK-SIAT1 is a derivative of the MDCK (Madin-Darby Canine Kidney) cell line stably transfected with the human SIAT1 (ST6Gal-I, $\alpha$2,6-sialyltransferase) gene. This cell line was engineered to address the limitations of parental MDCK cells in influenza research. Human influenza viruses preferentially bind to $\alpha$2,6-linked sialic acid receptors, whereas standard MDCK cells predominantly express $\alpha$2,3-linked receptors. By overexpressing SIAT1, MDCK-SIAT1 cells mimic the receptor distribution of the human upper respiratory tract, significantly enhancing the isolation rate and titer of human influenza viruses. It is an industry-standard model for influenza surveillance and vaccine research.

BioVector® MDCK-SIAT1 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® MDCK-SIAT1 犬肾上皮细胞系

• 母本细胞： MDCK (NBL-2)

• 物种： 犬 (Canine) / 人 (Human SIAT1 基因)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样，呈典型的铺路石状排列

2. 培养条件

• 基础培养基： BioVector® DMEM 或 MEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 筛选抗生素： 含有适量的 G418 (Geneticin)，以维持 SIAT1 基因的稳定表达。

• 培养环境： 37 摄氏度，5% CO2

• 传代比例： 1:3 至 1:6；建议在细胞汇合度达到 90% 时传代。

3. 细胞应用

• 病毒分离与鉴定： 特别是针对季节性流感病毒（A型和B型）的高效率分离。

• 抗病毒药物筛选： 评估神经氨酸酶抑制剂等药物对人流感病毒的抑制效果。

• 受体结合分析： 研究流感病毒血凝素 (HA) 与 $\alpha$2,6-唾液酸受体的相互作用。

4. 注意事项

• 受体完整性： 病毒感染实验前，需确保细胞培养基中不含外源神经氨酸酶。

• 维持抗性： 长期培养需添加 G418，否则 SIAT1 表达量可能会随代数增加而下降，导致对人流感病毒敏感性降低。

English Datasheet

1. General Product Information

• Product Name: BioVector® MDCK-SIAT1 Canine Kidney Epithelial Cell Line

• Parental Line: MDCK (NBL-2)

• Species: Canine (Parent) / Human (SIAT1 transgene)

• Growth Properties: Adherent

• Morphology: Epithelial-like, exhibiting a classic cobblestone appearance.

2. Culture Conditions

• Basal Medium: BioVector® DMEM or MEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Selection Marker: Supplemented with G418 (Geneticin) to ensure stable maintenance of the human SIAT1 expression cassette.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 90% confluence.

3. Applications

• Virus Isolation and Characterization: Highly efficient isolation of seasonal human influenza A and B viruses.

• Antiviral Screening: Evaluating the efficacy of neuraminidase inhibitors and other candidates against humanized viral targets.

• Binding Assays: Studying the interaction between influenza Hemagglutinin (HA) and $\alpha$2,6-linked sialic acid receptors.

4. Key Usage Notes

• Selection Pressure: Continuous cultivation requires G418 to prevent loss of the SIAT1 transgene, which would reduce the cell's sensitivity to human influenza strains.

• Infection Protocols: For influenza infection, cells must be washed to remove serum (which contains inhibitors) and supplemented with TPCK-treated trypsin to facilitate viral entry.

注意 / Note: BioVector® MDCK-SIAT1 应在生物安全二级 (BSL-2) 条件下操作。为保证病毒滴度，建议使用低代数细胞进行感染实验。

BioVector® MDCK-SIAT1 should be handled under Biosafety Level 2 (BSL-2) conditions. To ensure high viral titers, BioVector® recommends using low-passage cells for all infection studies.

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E-mail: BioVector@163.com

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