BioVector® pYTU植物基因编辑载体 / pYTU Vector

通用定义 / General Definition:BioVector® pYTU 是一种专门用于植物合成生物学和基因编辑的二元表达载体。它是 pY 系列载体（如 pYUU, pYTU）的成员，通常基于 pCAMBIA 骨架构建。pYTU 的核心设计目的是通过 Agrobacterium（农杆菌） 介导的转化，在植物中实现基因的高效表达或 CRISPR/Cas9 介导的基因组编辑。其名称中的 "U" 通常代表 Ubiquitin（泛素）启动子，用于驱动核心组件（如 Cas9 或目的基因）在植物组织中的组成型强表达。

BioVector® pYTU is a binary expression vector specifically designed for plant synthetic biology and genome editing. As a member of the pY vector series (e.g., pYUU, pYTU), it is typically built upon the pCAMBIA backbone. The primary purpose of pYTU is to facilitate efficient gene expression or CRISPR/Cas9-mediated genome editing in plants via Agrobacterium-mediated transformation. The "U" in its designation often signifies the use of a Ubiquitin promoter to drive the constitutive high-level expression of core components, such as Cas9 or the gene of interest (GOI), across various plant tissues.

BioVector® pYTU 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® pYTU 植物表达载体

• 载体类型： 二元载体 (Binary Vector)

• 启动子： Ubiquitin (泛素启动子，通常为 Arabidopsis 或 Maize 来源)

• 应用领域： 植物基因过表达、CRISPR/Cas9 基因编辑、合成生物学元件组装

• 抗性基因： * 大肠杆菌/农杆菌： 卡那霉素 (Kanamycin) 或 大观霉素 (Spectinomycin)

  • 植物筛选： 潮霉素 (Hygromycin) 或 除草剂 (Basta/PPT)；部分版本支持 YFP 种子荧光筛选

2. 核心元件功能

• LB/RB 边界： T-DNA 左右边界，确保外源片段精确整合进植物基因组。

  
  

• Cas9/目的基因框： 由强 Ubiquitin 启动子驱动，适用于双子叶和单子叶植物。

• gRNA 表达框： 通常携带 U6 或 U3 启动子，用于驱动 CRISPR 引导 RNA。

3. 转化条件

• 大肠杆菌宿主： BioVector® DH5-alpha 或 Stbl3。

• 农杆菌宿主： BioVector® EHA105, LBA4404 或 GV3101。

English Datasheet

1. General Product Information

• Product Name: BioVector® pYTU Plant Expression Vector

• Vector Type: Binary Vector

• Promoter: Ubiquitin (commonly sourced from Arabidopsis or Maize)

• Applications: Plant gene overexpression, CRISPR/Cas9 genome editing, and synthetic biology circuit assembly.

• Selection Markers: * Bacterial (E. coli/Agro): Kanamycin or Spectinomycin.

  • Plant Selection: Hygromycin or Basta (PPT); certain variants support YFP fluorescence-based seed selection.

2. Core Element Functions

• LB/RB Borders: T-DNA Left and Right Borders, ensuring precise integration of the DNA fragment into the plant genome.

• Expression Cassette: Driven by a robust Ubiquitin promoter, making it suitable for high-level expression in both dicots and monocots.

• gRNA Module: Typically contains a U6 or U3 promoter for driving the expression of CRISPR guide RNAs.

3. Transformation Guidelines

• E. coli Strains: BioVector® DH5-alpha or Stbl3.

• Agrobacterium Strains: BioVector® EHA105, LBA4404, or GV3101.

Note: For CRISPR applications, ensure that the gRNA target sequence is correctly cloned into the pYTU scaffold. BioVector® recommends verifying the final construct via sequencing across the promoter-insert junction. When using YFP-based selection, high-resolution fluorescence microscopy is required for identifying transgenic seeds.

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