BioVector® SL-15 人鼻咽癌细胞系 / SL-15 Human Nasopharyngeal Carcinoma Cell Line

通用定义 / General Definition:BioVector® SL-15 是一种源自人类鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮细胞系。它是研究鼻咽癌生物学行为的重要体外模型之一。SL-15 细胞常被用于探索 NPC 的发病机制、肿瘤细胞的迁移与侵袭能力、以及对新型抗癌药物（包括化疗药、靶向药物及天然产物提取物）的敏感性评价。在鼻咽癌研究领域，SL-15 与 CNE-1、CNE-2 等细胞系共同构成了研究东亚高发性鼻咽恶性肿瘤的基础工具。

BioVector® SL-15 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It serves as a significant in vitro model for investigating the biological characteristics of NPC. SL-15 cells are frequently employed to explore the pathogenesis of NPC, cell migration and invasion capabilities, and the sensitivity to novel anti-cancer agents, including chemotherapeutics, targeted drugs, and natural product extracts. In the field of nasopharyngeal cancer research, SL-15, along with lines like CNE-1 and CNE-2, forms the fundamental toolkit for studying this malignancy, which has a high incidence in East Asia.

BioVector® SL-15 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® SL-15 人鼻咽癌细胞

• 组织来源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，饱和湿度

• 传代比例： 1:3 至 1:4；建议在细胞汇合度达到 80% - 90% 时进行传代。

3. 细胞应用

• 抗肿瘤药物筛选： 评估小分子抑制剂对 NPC 细胞增殖和凋亡的影响。

• 信号转导研究： 分析调控鼻咽癌细胞恶性表型的关键信号通路（如 Wnt, NF-kB 等）。

• 放射增敏研究： 测试不同化合物对 SL-15 细胞放射治疗敏感性的增强作用。

4. 注意事项

• STR 鉴定： 鉴于 NPC 细胞系历史上存在交叉污染风险，BioVector® 强烈建议定期进行 STR 鉴定。

• 无菌操作： 严格遵循无菌技术，防止支原体及其他微生物污染。

English Datasheet

1. General Product Information

• Product Name: BioVector® SL-15 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach 80% - 90% confluence.

3. Applications

• Anti-tumor Drug Screening: Assessing the effects of small molecule inhibitors on NPC cell proliferation and apoptosis.

• Signal Transduction: Analyzing key signaling pathways (e.g., Wnt, NF-kB) that regulate the malignant phenotype of nasopharyngeal cancer cells.

• Radiosensitization: Testing the ability of various compounds to enhance the sensitivity of SL-15 cells to radiation therapy.

4. Key Usage Notes

• Cell Authentication: Given the historical risks of cross-contamination among NPC cell lines, BioVector® strongly recommends periodic STR (Short Tandem Repeat) profiling.

• Aseptic Technique: Maintain strict sterile procedures to prevent Mycoplasma and other microbial contaminations.

Note: SL-15 cells should be handled under Biosafety Level 2 (BSL-2) conditions. For optimal experimental results, BioVector® recommends the use of validated culture media and high-grade FBS to ensure consistent cell growth and phenotypic stability.

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