BioVector® Maska-98 人皮肤鳞状细胞癌细胞系 / Maska-98 Human Cutaneous Squamous Cell Carcinoma Cell Line

通用定义 / General Definition:BioVector® Maska-98 是一种源自人类皮肤鳞状细胞癌 (Cutaneous Squamous Cell Carcinoma, cSCC) 的细胞系。cSCC 是全球第二常见的皮肤癌，具有潜在的局部侵袭和远处转移能力。Maska-98 细胞系常被用于研究皮肤肿瘤的发生、表皮细胞的异常分化、肿瘤微环境相互作用以及评估针对非黑色素瘤皮肤癌的新型化疗药物、光动力疗法 (PDT) 和免疫检查点抑制剂的药效。

BioVector® Maska-98 is a cell line derived from Human Cutaneous Squamous Cell Carcinoma (cSCC). cSCC is the second most common form of skin cancer worldwide, characterized by its potential for local invasion and distant metastasis. The Maska-98 cell line is frequently utilized in studies concerning skin oncogenesis, aberrant epidermal differentiation, tumor microenvironment interactions, and the evaluation of novel chemotherapeutics, Photodynamic Therapy (PDT), and immune checkpoint inhibitors specifically for non-melanoma skin cancers.

BioVector® Maska-98 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® Maska-98 人皮肤鳞状细胞癌细胞

• 组织来源： 皮肤 (Skin)

• 疾病背景： 鳞状细胞癌 (SCC)

• 生长特性： 贴壁生长

• 细胞形态： 多角形，典型的上皮样形态，常呈成团或岛状生长

2. 培养条件

• 基础培养基： BioVector® DMEM/F12 或 RPMI-1640 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 关键添加剂： 根据具体研究需要，可能需添加氢化可的松或表皮生长因子 (EGF) 以维持其上皮特性。

• 培养环境： 37 摄氏度，5% CO2

• 传代比例： 1:3 至 1:4；建议在细胞汇合度达到 80%-90% 时进行传代。

3. 细胞应用

• 致癌机制研究： 探索紫外线损伤或特定基因突变（如 TP53, NOTCH）在皮肤癌发展中的作用。

• 屏障与浸润： 研究 SCC 细胞如何突破基底膜并向真皮层浸润。

• 局部治疗评估： 测试外用药物或物理疗法对皮肤肿瘤细胞的杀伤效率。

4. 注意事项

• 细胞接触抑制： 鳞癌细胞在汇合度过高时可能出现分化迹象，影响实验一致性，建议及时传代。

• 操作防护： 尽管是贴壁细胞，但在消化过程中应避免剧烈吹打以保持细胞活力。

English Datasheet

1. General Product Information

• Product Name: BioVector® Maska-98 Human Cutaneous Squamous Cell Carcinoma Cell Line

• Tissue Source: Skin

• Disease Context: Squamous Cell Carcinoma (SCC)

• Growth Properties: Adherent

• Morphology: Polygonal, typical epithelial morphology, often growing in clusters or "islands."

2. Culture Conditions

• Basal Medium: BioVector® DMEM/F12 or RPMI-1640 Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Supplements: Depending on specific research requirements, Hydrocortisone or EGF may be added to maintain the epithelial phenotype.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Oncogenesis Mechanisms: Exploring the roles of UV damage or specific mutations (e.g., TP53, NOTCH) in the progression of skin cancer.

• Invasion Studies: Investigating how SCC cells breach the basement membrane and infiltrate the dermal layers.

• Topical Therapy Assessment: Testing the efficiency of topical pharmacological agents or physical modalities (like PDT) in eradicating skin tumor cells.

4. Key Usage Notes

• Confluence Management: SCC cells may initiate differentiation pathways if allowed to overgrow; maintain timely subculturing to ensure experimental consistency.

• Standard Precautions: Handle under Biosafety Level 2 (BSL-2) conditions. BioVector® recommends the use of validated high-grade FBS to support the robust growth of the Maska-98 line.

Note: For research involving skin barrier modeling, BioVector® Maska-98 can be used in 3D organotypic cultures. To ensure the authenticity of your results, BioVector® advises regular STR profiling and mycoplasma screening.

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