BioVector® MUG-CCArl 人透明细胞软骨肉瘤细胞系 / MUG-CCArl Human Clear Cell Chondrosarcoma Cell Line

通用定义 / General Definition:BioVector® MUG-CCArl 是一种极其罕见且具有高度专业化用途的人类透明细胞软骨肉瘤 (Clear Cell Chondrosarcoma, CCC) 细胞系。透明细胞软骨肉瘤是软骨肉瘤的一种罕见亚型，临床特征是生长缓慢但具有局部侵袭性。MUG-CCArl 细胞系由奥地利格拉茨医学院（Medical University of Graz）建立，为研究该罕见肿瘤的分子发病机制、筛选潜在的靶向药物（如针对表观遗传调控或代谢途径的药物）以及鉴定新的生物标志物提供了宝贵的体外工具。

BioVector® MUG-CCArl is an extremely rare and highly specialized Human Clear Cell Chondrosarcoma (CCC) cell line. Clear cell chondrosarcoma is an uncommon subtype of chondrosarcoma characterized clinically by slow growth but local invasiveness. The MUG-CCArl cell line was established by the Medical University of Graz, providing an invaluable in vitro tool for investigating the molecular pathogenesis of this rare malignancy, screening potential targeted therapies (such as those targeting epigenetic regulation or metabolic pathways), and identifying novel biomarkers.

BioVector® MUG-CCArl 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® MUG-CCArl 人透明细胞软骨肉瘤细胞

• 组织来源： 骨 (Bone) / 软骨 (Cartilage)

• 疾病背景： 透明细胞软骨肉瘤 (CCC)

• 生长特性： 贴壁生长

• 细胞形态： 呈现多角形或纺锤形，胞质丰富，常见透明细胞特征

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 与 DMEM (1:1) 混合培养基 或 特制的骨肉瘤培养基

• 血清添加： 10%-15% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，饱和湿度

• 传代比例： 1:2 至 1:3；由于该细胞生长速度可能较慢，建议在汇合度达到 80% 时进行传代

3. 细胞应用

• 罕见肿瘤研究： 探索导致软骨肉瘤细胞出现“透明细胞”形态的代谢异常。

• 精准医疗： 评估针对特定基因突变或表观遗传改变的抑制剂效果。

• 分化研究： 研究恶性软骨细胞的分化障碍及其逆转可能性。

4. 注意事项

• 倍增时间： 罕见肿瘤细胞系的倍增时间可能较长，培养时需耐心观察，避免频繁传代干扰细胞状态。

• 基质相互作用： 软骨来源细胞对细胞外基质 (ECM) 较敏感，使用 BioVector® 胶原蛋白涂层器皿可能有助于提高细胞附着率。

English Datasheet

1. General Product Information

• Product Name: BioVector® MUG-CCArl Human Clear Cell Chondrosarcoma Cell Line

• Tissue Source: Bone / Cartilage

• Disease Context: Clear Cell Chondrosarcoma (CCC)

• Growth Properties: Adherent

• Morphology: Polygonal or spindle-shaped with abundant cytoplasm, exhibiting characteristic "clear cell" features.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 and DMEM (1:1) mixture or specialized sarcoma medium.

• Serum Supplement: 10%-15% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:2 to 1:3 ratio; as rare tumor lines often grow slowly, subculture when cells reach 80% confluence.

3. Applications

• Rare Tumor Research: Investigating the metabolic aberrations leading to the "clear cell" morphology in chondrosarcoma cells.

• Precision Medicine: Evaluating the efficacy of inhibitors targeting specific genetic mutations or epigenetic alterations.

• Differentiation Dynamics: Studying the differentiation blocks in malignant chondrocytes and the potential for their reversal.

4. Key Usage Notes

• Doubling Time: Be aware that the doubling time for this rare cell line may be longer than standard lines; patience is required to avoid over-handling.

• Matrix Sensitivity: Since these cells are derived from cartilage, they may be sensitive to the extracellular matrix (ECM). Using BioVector® Collagen-coated flasks may enhance attachment and growth.

Note: Due to the rarity of MUG-CCArl, it is essential to perform regular identity verification via STR profiling. Handle under Biosafety Level 2 (BSL-2) conditions. BioVector® recommends the use of high-quality FBS and specialized media to maintain the unique clear cell phenotype.

Supplier：

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net