BioVector® pAJM.773细菌生物传感器质粒 / pAJM.773 Bacterial Biosensor Plasmid

通用定义 / General Definition:

BioVector® pAJM.773 是一种源自麻省理工学院 (MIT) Voigt 实验室 Marionette 传感器集合 的合成生物学载体。该质粒专门用于在非 Marionette 标准大肠杆菌株中构建高效的香草酸 (Vanillic acid / Van) 生物传感器。它是一个“全合一” (All-in-one) 载体，同时编码了转录阻遏蛋白 VanR-AM 和受其调控的 PVanCC-YFP 报告模块。当环境中存在香草酸时，VanR 蛋白发生变构并从启动子上解离，从而诱导黄色荧光蛋白 (YFP) 的表达。该质粒是研究小分子感应、代谢工程控制以及复杂合成基因线路的基础元件。

BioVector® pAJM.773 is a synthetic biology vector from the Marionette Sensor Collection, developed by the Voigt Lab at MIT. This plasmid is specifically designed to function as a high-performance Vanillic acid (Van) biosensor in standard (non-Marionette) E. coli strains. It is an "all-in-one" vector, encoding both the transcriptional repressor VanR-AM and the corresponding PVanCC-YFP reporter module. In the presence of vanillic acid, the VanR protein undergoes a conformational change and dissociates from the promoter, thereby inducing the expression of Yellow Fluorescent Protein (YFP). This plasmid is a fundamental component for small-molecule sensing, metabolic engineering control, and complex synthetic genetic circuit design.

BioVector® pAJM.773 技术说明书 (Plasmid Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® pAJM.773 传感器质粒

• Addgene 编号： 108527

  
  

• 感应诱导剂： 香草酸 (Vanillic acid, Van)

  
  

• 报告基因： EYFP (增强型黄色荧光蛋白)

  
  

• 抗性基因： 卡那霉素 (Kanamycin)

  
  

• 复制子： p15A (低拷贝，约 10-15 copies/cell)

2. 培养与扩增条件

• 宿主菌： BioVector® NEB Stable 或 DH5-alpha。

• 培养基： BioVector® LB 培养基。

• 抗生素： 50 ug/mL 卡那霉素。

  
  

• 环境： 37 摄氏度，恒温振荡。

3. 载体应用

• 小分子检测： 在大肠杆菌中实时定量监测香草酸的浓度。

• 动态代谢控制： 利用环境中的代谢产物作为开关，自动调节下游代谢途径的基因表达。

• 生物逻辑门： 作为 Marionette 系统的 12 个正交传感器之一，用于构建多输入逻辑线路。

4. 注意事项

• 宿主要求： 即使在普通的非 Marionette 菌株中也能工作，因为它自带了阻遏蛋白表达框。

• 低拷贝优势： 采用 p15A 复制子是为了降低漏表达 (Leaky expression) 并减轻细胞代谢负担。

English Datasheet

1. General Product Information

• Product Name: BioVector® pAJM.773 Sensor Plasmid

• Addgene ID: 108527

  
  

• Inducer: Vanillic acid (Van)

• Reporter Gene: EYFP (Enhanced Yellow Fluorescent Protein)

  
  

• Bacterial Resistance: Kanamycin (50 ug/mL)

  
  

• Replicon: p15A (Low-copy, approx. 10-15 copies/cell)

2. Culture and Amplification Conditions

• Host Strains: BioVector® NEB Stable or DH5-alpha.

• Culture Medium: BioVector® LB Medium.

• Incubation: 37 degrees Celsius with constant shaking.

3. Applications

• Small-Molecule Detection: Real-time quantitative monitoring of vanillic acid concentration in E. coli.

• Dynamic Metabolic Control: Using metabolic intermediates as triggers to automatically regulate gene expression in downstream pathways.

• Genetic Logic Gates: Serving as one of the 12 orthogonal sensors in the Marionette system for constructing multi-input genetic circuits.

  
  

4. Key Usage Notes

• Strain Compatibility: This vector is functional in standard E. coli strains because it contains its own constitutively expressed repressor (VanR).

• Low Leakage: The use of the p15A origin helps minimize basal (leaky) expression and reduces the metabolic burden on the host cell.

Note: For optimal sensor performance with low background noise, BioVector® recommends using M9 minimal media supplemented with necessary carbon sources. Always verify the induction range and sensitivity of pAJM.773 in your specific host strain using BioVector® high-purity Vanillic acid inducer solutions.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net