BioVector® NHEK 原代正常人表皮角质形成细胞 / Primary Normal Human Epidermal Keratinocytes (NHEK)

通用定义 / General Definition:BioVector® 原代正常人表皮角质形成细胞 (NHEK) 是直接从人类皮肤组织（通常为包皮或手术切除的皮肤）中分离出来的细胞。角质形成细胞是表皮的主要构成细胞，占表皮细胞总数的 90% 以上。在体外培养中，这些细胞保持了其在体内的生物学特性，包括增殖能力和向角质层分化的潜能。它们是研究皮肤屏障功能、创伤愈合、炎症反应、皮肤毒理学以及化妆品功效测试的核心细胞模型。

BioVector® Primary Normal Human Epidermal Keratinocytes (NHEK) are cells isolated directly from human skin tissue (typically neonatal foreskin or adult surgical specimens). Keratinocytes are the predominant cell type in the epidermis, accounting for over 90% of the total epidermal cell population. In vitro, these cells retain their native biological characteristics, including proliferative capacity and the potential to differentiate into the cornified layer. They serve as a fundamental cellular model for studying skin barrier function, wound healing, inflammatory responses, skin toxicology, and cosmetic efficacy testing.

BioVector® NHEK 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® 原代正常人表皮角质形成细胞

• 细胞来源： 人类皮肤组织 (Human Skin)

• 生长特性： 贴壁生长

• 细胞形态： 鹅卵石样、典型上皮形态

• 生物安全性： 所有供体组织均经过 HIV, HBV, HCV 等病毒检测，结果均为阴性。

2. 培养条件

• 基础培养基： BioVector® 角质形成细胞专用无血清培养基 (Keratinocyte Growth Medium, KGM)

• 关键添加剂： BPE (牛垂体提取物)、EGF (表皮生长因子)、胰岛素、氢化可的松。

• 培养环境： 37 摄氏度，5% CO2

• 传代比例： 1:2 至 1:4；建议在细胞汇合度达到 70% 至 80% 时传代。注意： 原代细胞不建议培养至 100% 汇合，否则会导致不可逆的分化。

3. 细胞应用

• 皮肤替代模型： 构建 3D 有组织表皮模型 (RHE)。

• 屏障功能研究： 检测细胞间紧密连接蛋白 (Tight Junctions) 的表达。

• 临床前测试： 评估药物或护肤品成分的刺激性、过敏性和吸收率。

4. 注意事项

• 钙离子浓度： 角质形成细胞对钙离子浓度极度敏感。低钙培养基维持其增殖，高钙诱导其分化。

• 有限寿命： 原代细胞具有有限的倍增次数（通常可传 3 至 5 代），随着代数增加，细胞会逐渐衰老。

English Datasheet

1. General Product Information

• Product Name: BioVector® Primary Normal Human Epidermal Keratinocytes (NHEK)

• Organism: Human Skin Tissue

• Growth Properties: Adherent

• Morphology: Cobblestone-like, typical epithelial morphology

• Biosafety: All donor tissues are screened and negative for HIV-1, HBV, and HCV.

2. Culture Conditions

• Basal Medium: BioVector® Keratinocyte Growth Medium (KGM, Serum-Free)

• Key Supplements: BPE (Bovine Pituitary Extract), EGF, Insulin, and Hydrocortisone.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:2 to 1:4 ratio; subculture at 70% to 80% confluence. Crucial: Never allow primary keratinocytes to reach 100% confluence as this triggers irreversible terminal differentiation.

3. Applications

• Skin Equivalents: Construction of 3D reconstituted human epidermis (RHE) models.

• Barrier Function: Studying the expression of tight junction proteins and lipid metabolism.

• Preclinical Testing: Assessing irritation, sensitization, and absorption of pharmacological or cosmetic compounds.

4. Key Usage Notes

• Calcium Sensitivity: Keratinocyte behavior is strictly regulated by calcium levels. Low calcium concentrations promote proliferation, while higher concentrations (e.g., above 0.1 mM) induce differentiation.

• Finite Lifespan: As primary cells, NHEKs have a limited doubling capacity (typically 3 to 5 passages). Cellular senescence increases with each passage.

Note: To maintain the undifferentiated, proliferative state of BioVector® NHEK cells, it is essential to use BioVector® specialized Serum-Free Medium and minimize exposure to proteolytic enzymes during subculturing. Handle cells with extreme care to ensure high attachment efficiency post-thaw.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net