BioVector® pSAT1-mVenus-C (pE3228) 植物荧光表达载体 / pSAT1-mVenus-C (pE3228) Plant Fluorescence Expression Vector

通用定义 / General Definition:BioVector® pSAT1-mVenus-C (pE3228) 是一种用于植物系统的卫星载体 (Satellite Vector)，属于广泛使用的 pSAT 系列。该载体设计用于在目的蛋白的 C 端 (C-terminus) 融合表达 mVenus（一种单体、增强型黄色荧光蛋白变体）。mVenus 具有极快的成熟速度和更强的抗酸性，使其成为活体植物成像、蛋白质定位研究以及双分子荧光互补 (BiFC) 实验的卓越工具。作为 pSAT 载体，它通常与 pPZP 或 pPZP-RCS 模块化系统配合使用，通过罕见限制性内切酶（如 AscI）将整个表达盒转移到植物二元载体中。

BioVector® pSAT1-mVenus-C (pE3228) is a satellite vector designed for plant systems, belonging to the widely used pSAT series. This vector is engineered to express the mVenus protein (a monomeric, enhanced yellow fluorescent protein variant) fused to the C-terminus of a target protein. mVenus is characterized by its rapid maturation and improved acid stability, making it an excellent tool for live plant imaging, protein localization studies, and Bimolecular Fluorescence Complementation (BiFC) assays. As a pSAT vector, it is typically used in conjunction with the pPZP or pPZP-RCS modular systems, allowing the entire expression cassette to be transferred to a plant binary vector via rare-cutting restriction enzymes like AscI.

BioVector® pSAT1-mVenus-C (pE3228) 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® pSAT1-mVenus-C 表达载体

• 载体编号： pE3228

• 启动子： 2x35S 启动子（增强型花椰菜花叶病毒 35S 启动子）

• 荧光标签： mVenus (C 端融合)

• 抗性基因： 氨苄青霉素 (Ampicillin)

• 终止子： TL 终止子 (Agrobacterium tumefaciens)

2. 培养与扩增条件

• 宿主菌： BioVector® DH5-alpha 或 TOP10。

• 培养基： BioVector® LB 培养基。

• 抗生素： 100 ug/mL 氨苄青霉素。

• 环境： 37 摄氏度，恒温振荡。

3. 载体应用

• 亚细胞定位： 通过共聚焦显微镜观察目的蛋白在植物细胞内的分布。

• 多色成像： mVenus 的光谱特性允许其与青色 (CFP) 或红色 (RFP) 荧光蛋白结合使用。

• BiFC 实验： 作为 Venus 片段供体，研究植物体内的蛋白质相互作用。

4. 注意事项

• 移码风险： 在克隆目的基因时，请务必核对序列，确保目的基因与 C 端的 mVenus 保持正确的读码框。

• 模块化转移： pSAT1 表达盒侧翼通常带有罕见酶切位点（如 AscI），便于整体迁移至二元载体进行农杆菌转化。

English Datasheet

1. General Product Information

• Product Name: BioVector® pSAT1-mVenus-C Expression Vector

• Vector ID: pE3228

• Promoter: Double 35S Promoter (2x35S CaMV)

• Fluorescent Tag: mVenus (C-terminal fusion)

• Antibiotic Resistance: Ampicillin (100 ug/mL)

• Terminator: TL Terminator

2. Culture and Amplification Conditions

• Host Strains: BioVector® DH5-alpha or TOP10.

• Culture Medium: BioVector® LB Medium.

• Incubation: 37 degrees Celsius with constant shaking.

3. Applications

• Subcellular Localization: Visualizing the spatial distribution of target proteins within plant cells via confocal microscopy.

• Multi-color Imaging: The spectral properties of mVenus allow for co-imaging with Cyan (CFP) or Red (RFP) fluorescent proteins.

• Bimolecular Fluorescence Complementation (BiFC): Utilizing Venus fragments for investigating protein-protein interactions in planta.

4. Key Usage Notes

• Frame Alignment: When cloning your Gene of Interest (GOI), ensure there are no internal stop codons and that the GOI is in-frame with the C-terminal mVenus tag.

• Vector Mobilization: The pSAT1 expression cassette is typically flanked by rare restriction sites (e.g., AscI). This allows for easy shuttling into a plant binary vector (such as the pPZP-RCS series) for Agrobacterium-mediated transformation.

Note: To achieve the best imaging results in leaves or protoplasts, BioVector® recommends using high-purity plasmid DNA extracted with BioVector® Endotoxin-Free Kits. For BiFC experiments, verify the orientation and junction sequences using BioVector® sequence validation services.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net