BioVector® Jurkat 76 人类 T 淋巴细胞缺陷型细胞系 / Jurkat 76 Human T-Lymphocyte Deficient Cell Line

通用定义 / General Definition:

BioVector® Jurkat 76 是经典 Jurkat 细胞系的一个特殊衍生亚型。该细胞系的独特之处在于它是 $TCR$（T 细胞受体）和 $CD3$ 表达缺陷型。由于它不表达内源性 $TCR$$\alpha$ 和 $\beta$ 链，因此不会产生内源性 TCR 复合物的干扰。这使得 Jurkat 76 成为 TCR 基因转导、TCR 结构与功能研究、以及新型 TCR-T 免疫疗法筛选的理想“空白受体”模型。

BioVector® Jurkat 76 is a specialized derivative of the classic Jurkat cell line. Its defining characteristic is the lack of endogenous $TCR$ (T-cell receptor) and $CD3$ expression. Because it does not produce endogenous TCR $\alpha$ and $\beta$ chains, there is no interference from native TCR complexes. This makes Jurkat 76 an ideal "blank slate" model for TCR gene transduction, studies on TCR structure and function, and the screening of novel TCR-T immunotherapies.

BioVector® Jurkat 76 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® Jurkat 76 人 T 淋巴细胞缺陷型细胞

• 细胞来源： 人类 (Human)

• 组织来源： 外周血 (Peripheral Blood / T-cell Leukemia)

• 生长特性： 悬浮生长 (Suspension)

• 细胞形态： 淋巴母细胞样

2. 培养条件

• 基础培养基： BioVector® RPMI 1640 培养基

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 培养环境： 37°C，5% $CO_2$

3. 细胞应用

• TCR 重组研究： 转染外源性 TCR 基因以研究特定的抗原识别。

• 抗原提呈验证： 与表达特定 pMHC 的靶细胞共培养，检测激活信号。

• 信号转导机制： 研究 TCR 缺失背景下，其他共刺激分子或细胞因子受体的功能。

4. 注意事项

• 细胞密度： 建议维持密度在 $1 \times 10^5$ 至 $1 \times 10^6$ cells/mL 之间。悬浮细胞对营养消耗较快，需定期观察。

• 缺陷验证： 在进行 TCR 转染实验前，建议通过流式细胞术 (FACS) 再次确认 CD3/TCR 的阴性背景。

English Datasheet

1. General Product Information

• Product Name: BioVector® Jurkat 76 Human T-Lymphocyte Deficient Cell Line

• Organism: Human

• Tissue Source: Peripheral Blood (T-cell Leukemia)

• Growth Properties: Suspension

• Morphology: Lymphoblast-like

2. Culture Conditions

• Basal Medium: BioVector® RPMI 1640 Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$

3. Applications

• TCR Reconstitution: Transfecting exogenous TCR genes to study specific antigen recognition without endogenous competition.

• Antigen Presentation Assays: Co-culturing with pMHC-expressing target cells to monitor downstream activation signals (e.g., IL-2 production).

• Signaling Research: Investigating the role of co-stimulatory molecules or cytokine receptors in a TCR-negative background.

4. Key Usage Notes

• Maintenance Density: Keep cell concentration between $1 \times 10^5$ and $1 \times 10^6$ cells/mL. Suspension cultures exhaust nutrients quickly and require regular monitoring.

• Phenotypic Confirmation: It is highly recommended to verify the CD3/TCR negative status via Flow Cytometry (FACS) prior to beginning any TCR reconstitution experiments to ensure the BioVector® Jurkat 76 "blank" background.

Note: Handle BioVector® Jurkat 76 cells under Biosafety Level 2 (BSL-2) standards. For optimal transfection efficiency of large TCR constructs, the use of BioVector® RPMI 1640 Medium and high-grade BioVector® Fetal Bovine Serum is essential.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net