BioVector® 普通拟杆菌 / Bacteroides vulgatus

通用定义 / General Definition:

BioVector® 普通拟杆菌 (Bacteroides vulgatus) 是一种革兰氏阴性、专性厌氧、不产生芽孢的杆状细菌。它是人类肠道菌群（核心菌群）中最丰富的成员之一。该菌在调节宿主免疫系统、维持肠道稳态、分解复杂多糖以及胆汁酸代谢中发挥着关键作用。在研究领域，它被广泛用于克罗恩病（CD）、溃疡性结肠炎（UC）等炎症性肠病（IBD）的发病机制研究，以及肠道微生物组对肥胖和代谢综合征的影响研究。

BioVector® Bacteroides vulgatus is a Gram-negative, obligately anaerobic, non-spore-forming, rod-shaped bacterium. It is one of the most abundant members of the human gut microbiome (core microbiota). This bacterium plays a critical role in modulating the host immune system, maintaining intestinal homeostasis, fermenting complex polysaccharides, and metabolizing bile acids. In research, it is widely utilized for studying the pathogenesis of Inflammatory Bowel Disease (IBD), such as Crohn's disease and ulcerative colitis, as well as the impact of the gut microbiome on obesity and metabolic syndrome.

BioVector® Bacteroides vulgatus 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® 普通拟杆菌

• 菌种学名：Bacteroides vulgatus

• 分类学： 拟杆菌门 (Bacteroidetes)，拟杆菌纲 (Bacteroidia)，拟杆菌目 (Bacteroidales)

• 生长特性： 专性厌氧

• 生物安全性： BSL-1/BSL-2（取决于特定菌株和应用）

2. 培养条件

• 基础培养基： BioVector® 强化梭菌培养基 (RCM) 或 BioVector® BHI 培养基（建议添加血红素和维生素 K1）

• 血清/添加剂： 5% - 10% BioVector® 脱纤维羊血（可选，用于增强生长）

• 培养环境： 37°C，绝对厌氧环境（如 80% $N_2$, 10% $CO_2$, 10% $H_2$）

3. 菌种应用

• 肠道稳态研究： 探索拟杆菌对肠道屏障功能的维护作用。

• 免疫免疫调节： 研究其与 T 细胞（特别是 Treg 和 Th17）的相互作用。

• 代谢组学研究： 纤维降解过程中的短链脂肪酸 (SCFA) 产量测定。

• IBD 模型： 作为条件致病菌或共生保护菌应用于小鼠结肠炎模型。

4. 注意事项

• 厌氧操作：Bacteroides vulgatus 对氧气极度敏感。从复苏到接种的所有操作应在厌氧操作箱中进行。

• 菌种鉴定： 建议使用 BioVector® 16S rRNA 测序或 MALDI-TOF MS 进行定期的菌株纯度验证。

English Datasheet

1. General Product Information

• Product Name: BioVector® Bacteroides vulgatus

• Species:Bacteroides vulgatus

• Taxonomy: Phylum Bacteroidetes, Class Bacteroidia, Order Bacteroidales

• Growth Properties: Obligate Anaerobe

• Biosafety Level: BSL-1 / BSL-2 (depending on strain and local regulations)

2. Culture Conditions

• Basal Medium: BioVector® Reinforced Clostridial Medium (RCM) or BioVector® BHI Medium (supplemented with Hemin and Vitamin K1)

• Supplements: 5% - 10% BioVector® Defibrinated Sheep Blood (optional for enhanced growth)

• Incubation: 37°C, Strict Anaerobic Conditions (e.g., 80% $N_2$, 10% $CO_2$, 10% $H_2$)

3. Applications

• Intestinal Homeostasis: Investigating the role of Bacteroides in maintaining gut barrier function.

• Immunomodulation: Studying interactions with T-cell populations (specifically Treg and Th17).

• Metabolomics: Measuring Short-Chain Fatty Acid (SCFA) production during fiber fermentation.

• IBD Models: Application in murine colitis models as either a pathobiont or a commensal protective agent.

4. Key Usage Notes

• Anaerobic Handling:Bacteroides vulgatus is highly sensitive to oxygen. All procedures from resuscitation to inoculation must be performed within an anaerobic chamber.

• Purity Validation: Periodic verification using BioVector® 16S rRNA sequencing or MALDI-TOF MS is recommended to ensure strain integrity.

Note: For optimal growth and metabolic activity, always use freshly prepared BioVector® Anaerobic Media. Proper use of BioVector® Hemin and Vitamin K1 supplements is critical for the robust expansion of Bacteroides vulgatus cultures.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net