BioVector® Met1 小鼠乳腺癌细胞系 / Met1 Mouse Mammary Carcinoma Cell Line

通用定义 / General Definition:

BioVector® Met1 是一种来源于 FVB/N 载体背景下 MMTV-PyMT 转基因小鼠自发性乳腺肿瘤的细胞系。该细胞系具有高度的侵袭性和转移潜力，主要表现为肺转移。在癌症研究中，BioVector® Met1 常被用作模拟人类乳腺癌进展、研究肿瘤血管生成以及评估针对转移性乳腺癌的新型疗法的经典模型。

BioVector® Met1 is a cell line derived from a spontaneous mammary tumor in an MMTV-PyMT transgenic mouse on an FVB/N background. Characterized by its high invasiveness and metastatic potential (primarily to the lungs), BioVector® Met1 is a well-established model in cancer research for mimicking human breast cancer progression, studying tumor angiogenesis, and evaluating novel therapeutics against metastatic disease.

BioVector® Met1 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® Met1 小鼠乳腺癌细胞

• 细胞来源： 小鼠 (FVB/N; MMTV-PyMT 转基因)

• 组织来源： 乳腺

• 生长特性： 贴壁生长

• 细胞形态： 上皮细胞样/多角形

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 培养环境： 37°C，5% $CO_2$，饱和湿度

3. 细胞应用

• 晚期乳腺癌进展与自发转移研究。

• 肿瘤血管生成及内皮细胞相互作用研究。

• 同种异体移植模型（用于 FVB 鼠的肿瘤免疫研究）。

4. 注意事项

• 高度恶性： BioVector® Met1 在体内生长迅速且具有极强的肺转移倾向，实验设计需考虑其较短的观察窗口期。

• 传代建议： 该细胞生长旺盛，建议在细胞汇合度达到 85% 时按 1:4 至 1:8 的比例进行传代。

English Datasheet

1. General Product Information

• Product Name: BioVector® Met1 Mouse Mammary Carcinoma Cell Line

• Organism: Mouse (FVB/N; MMTV-PyMT Transgenic)

• Tissue Source: Mammary Gland

• Growth Properties: Adherent

• Morphology: Epithelial-like / Polygonal

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Studies on advanced breast cancer progression and spontaneous metastasis.

• Research on tumor angiogenesis and interaction with endothelial cells.

• Syngeneic graft models (specifically for tumor immunology research in FVB mice).

4. Key Usage Notes

• Aggressive Phenotype: BioVector® Met1 grows rapidly in vivo and has a high propensity for lung metastasis; researchers should plan observation windows accordingly.

• Subculture: These cells are highly proliferative; it is recommended to subculture at 85% confluence with a split ratio of 1:4 to 1:8.

Note: Handle BioVector® Met1 cells strictly under Biosafety Level 2 (BSL-2) guidelines. To ensure experimental reproducibility and maintain the metastatic phenotype, the use of BioVector® DMEM Medium and BioVector® Fetal Bovine Serum is highly recommended.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail: BioVector@163.com

http://www.biovector.net