BioVector® D2.0R 小鼠乳腺癌休眠细胞系 / D2.0R Mouse Mammary Carcinoma Dormancy Cell Line

通用定义 / General Definition:

BioVector® D2.0R 是一种源自 BALB/c 小鼠自发性乳腺肿瘤的永久性细胞系。该细胞系在生物医学研究中具有独特地位，因为它在体外（In Vitro）能够正常增殖，但在植入体内（In Vivo）后会进入长时间的细胞周期停滞或休眠状态。这使其成为研究癌症远端转移、免疫逃逸及休眠唤醒机制的核心工具。

BioVector® D2.0R is a permanent cell line derived from a spontaneous mammary tumor in BALB/c mice. It holds a unique position in biomedical research due to its ability to proliferate normally In Vitro while entering a prolonged state of cell cycle arrest or dormancy upon In Vivo implantation. This makes it a pivotal tool for investigating cancer metastasis, immune evasion, and the mechanisms of dormancy awakening.

BioVector® D2.0R 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® D2.0R 小鼠乳腺癌休眠细胞

• 细胞来源： 小鼠 (BALB/c)

• 组织来源： 乳腺

• 生长特性： 贴壁生长

• 细胞形态： 上皮细胞样

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 培养环境： 37°C，5% $CO_2$，饱和湿度

3. 细胞应用

• 肿瘤细胞休眠与唤醒机制研究。

• 抗肿瘤药物筛选（特别是针对非增殖期微小残留病灶）。

• 肿瘤微环境与转移前生态位 (Pre-metastatic Niche) 研究。

4. 注意事项

• 表型转换： BioVector® D2.0R 细胞在体外培养时保持增殖，但在注入小鼠体内后表现出明显的休眠表型。

• 传代建议： 建议在细胞达到 80%-90% 汇合度时进行传代。

English Datasheet

1. General Product Information

• Product Name: BioVector® D2.0R Mouse Mammary Carcinoma Dormancy Cell Line

• Organism: Mouse (BALB/c)

• Tissue Source: Mammary Gland

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Mechanistic studies of tumor cell dormancy and reactivation.

• Anti-tumor drug screening (targeting non-proliferative Minimal Residual Disease).

• Research on tumor microenvironment and the pre-metastatic niche.

4. Key Usage Notes

• Phenotypic Switch: While BioVector® D2.0R cells proliferate actively in vitro, they exhibit a distinct dormant phenotype when injected in vivo.

• Subculture: It is recommended to subculture when the cell density reaches 80%-90% confluence.

Note: Always handle BioVector® D2.0R cells using standard Biosafety Level 2 (BSL-2) protocols. Consistent use of BioVector® DMEM Medium and BioVector® Fetal Bovine Serum is essential for maintaining phenotypic stability.

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E-mail: BioVector@163.com

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