LOX-IMVI BioVector®人黑色素瘤细胞株Human Melanoma Cell Line BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® LOX-IMVI 人黑色素瘤细胞系 / Human Melanoma Cell Line 数据说明书

产品名称: BioVector® LOX-IMVI 细胞
同义词: BioVector® LOXIMVI; BioVector® LOX IMVI; BioVector® LOX-IMVI; BioVector® LOX
中文名称: BioVector® LOX-IMVI 人黑色素瘤细胞系
英文名称: BioVector® LOX-IMVI Human Melanoma Cell Line
产品类别: 肿瘤细胞系；NCI-60 细胞系面板成员
物种来源: 人 (Homo sapiens)
组织来源: 皮肤；淋巴结转移灶
疾病: 恶性无色素性黑色素瘤 (Malignant Amelanotic Melanoma)
患者信息: 58岁高加索男性
构建方法: 从患者淋巴结转移灶获取肿瘤组织，经皮下接种至裸鼠建立异种移植物，随后建立体外细胞系
形态特性: 无色素性黑色素瘤细胞，无黑色素产生
生长特性: 贴壁生长
细胞标志物: 表达人黑色素瘤标志物 GD3 神经节苷脂
基因特征:

1. BRAF 突变: BRAF V600E 突变杂合子 (BRAF V600E heterozygous)

2. 染色体异常: Y 染色体缺失；7 号染色体三体 (Trisomy 7)

3. p53 状态: 野生型 p53 (wild type)

4. 突变负荷: 已报道 764 个突变

5. 拷贝数变异: 58 个基因扩增，543 个基因缺失
   特性特征:

6. NCI-60 成员: 作为美国国家癌症研究所 NCI-60 肿瘤细胞系面板的重要成员，被广泛用于抗肿瘤药物筛选和分子靶点鉴定

7. 高转移潜能: 在裸鼠中无论接种部位如何，均倾向于形成肺转移灶；接种后 3-5 周内可观察到实验动物死亡

8. 无色素性: 细胞不产生黑色素，呈无色素性表型

9. 转移研究模型: 是研究肿瘤转移机制和评估化疗药物疗效的优良体外模型

10. 药物敏感性: 已被广泛用于多种抗肿瘤化合物的敏感性测试，数据可公开获取
    研究应用:

11. 黑色素瘤转移机制研究

12. 抗肿瘤药物筛选与药效评估

13. BRAF V600E 突变相关靶向治疗研究

14. 肿瘤转移异种移植模型建立

15. 肿瘤细胞化疗敏感性研究
    培养基: BioVector® LOX-IMVI 细胞专用完全培养基（推荐基础成分：RPMI-1640 培养基，添加 5-10% 胎牛血清、2mM L-谷氨酰胺、1% 青霉素-链霉素）
    培养条件: 气相：95% 空气，5% 二氧化碳；温度：37°C
    传代方法: 当细胞融合度达到 80% 时，推荐使用 BioVector® 专用传代试剂（如 0.25% 胰蛋白酶-EDTA）进行消化，按 1:3 至 1:6 比例进行传代；解冻后可稳定培养至少 10 代而不影响细胞标志物表达和功能
    冻存液: BioVector® 细胞冻存液（推荐配方：90% 胎牛血清 + 10% DMSO）
    质量控制:

• 支原体检测：阴性

• 无菌检测：阴性

• 病毒检测：HPV-16、HPV-18、甲肝病毒、乙肝病毒、丙肝病毒、HIV-1、HIV-2 检测均为阴性

• 种属鉴定：经 STR 分型验证为人源，无大鼠、小鼠、中国仓鼠、金黄地鼠及非人灵长类交叉污染
  储存与运输: 液氮或干冰运输与保存
  生物安全等级: 1（需在二级生物安全柜内操作）
  仅供科研使用

English:
BioVector® LOX-IMVI Human Melanoma Cell Line Datasheet

Product Name: BioVector® LOX-IMVI Cells
Synonyms: BioVector® LOXIMVI; BioVector® LOX IMVI; BioVector® LOX-IMVI; BioVector® LOX
Chinese Name: BioVector® LOX-IMVI 人黑色素瘤细胞系
English Name: BioVector® LOX-IMVI Human Melanoma Cell Line
Product Category: Cancer Cell Line; NCI-60 Cell Line Panel Member
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Skin; Lymph node metastasis
Disease: Malignant Amelanotic Melanoma
Patient Information: 58-year-old Caucasian male
Derivation: Established from a lymph node metastasis of a patient, passaged as a subcutaneous xenograft in nude mice, and subsequently established as an in vitro cell line
Morphology: Amelanotic melanoma cells, no melanin production
Growth Characteristics: Adherent
Cell Markers: Expresses human melanoma marker GD3 ganglioside
Genetic Features:

1. BRAF Mutation: BRAF V600E heterozygous

2. Chromosomal Abnormalities: Loss of Y chromosome; Trisomy 7

3. p53 Status: Wild type

4. Mutation Burden: 764 mutations reported

5. Copy Number Variations: 58 genes with gain, 543 genes with loss
   Characteristics:

6. NCI-60 Member: As a key member of the National Cancer Institute's NCI-60 tumor cell line panel, widely used for anti-cancer drug screening and molecular target identification

7. High Metastatic Potential: Exhibits a tendency to form lung metastases in nude mice independent of inoculation site; mortality observed within 3-5 weeks post-injection

8. Amelanotic: Cells do not produce melanin, exhibiting an amelanotic phenotype

9. Metastasis Model: An excellent in vitro model for studying tumor metastasis mechanisms and evaluating chemotherapy efficacy

10. Drug Sensitivity: Extensively tested for sensitivity to various anti-cancer compounds with publicly available data
    Research Applications:

11. Melanoma metastasis mechanism studies

12. Anti-cancer drug screening and efficacy evaluation

13. BRAF V600E mutation-targeted therapy research

14. Establishment of tumor metastasis xenograft models

15. Tumor cell chemosensitivity studies
    Culture Medium: BioVector® Complete Medium for LOX-IMVI Cells (Recommended composition: RPMI-1640 medium supplemented with 5-10% fetal bovine serum, 2mM L-glutamine, 1% penicillin-streptomycin)
    Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
    Subculturing Method: When cells reach 80% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:3 to 1:6. Cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality
    Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
    Quality Control:

• Mycoplasma: Negative

• Sterility: Negative

• Virus Testing: Negative for HPV-16, HPV-18, Hepatitis A, B, C, HIV-1, and HIV-2

• Species Verification: STR profiling confirmed human origin; negative for cross-contamination from rat, mouse, Chinese hamster, Golden Syrian hamster, and non-human primate
  Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
  Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
  For Research Use Only

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