Vk12598 BioVector®小鼠骨髓瘤细胞系 / Mouse Myeloma Cell Line BioVector NTCC典型培养物保藏中心

BioVector® Vk12598 小鼠骨髓瘤细胞系 / Mouse Myeloma Cell Line 数据说明书

产品名称: BioVector® Vk12598 细胞
同义词: BioVector® Vk12598 骨髓瘤细胞
中文名称: BioVector® Vk12598 小鼠骨髓瘤细胞系
英文名称: BioVector® Vk12598 Mouse Myeloma Cell Line
产品类别: 肿瘤细胞系；多发性骨髓瘤模型
物种来源: 小鼠 (Mus musculus)，C57BL/6 背景 -1-4
组织来源: 脾脏 (从移植荷瘤小鼠脾脏分离) -1-5
细胞类型: 浆细胞 (Plasma cell) -1
疾病: 多发性骨髓瘤 (Multiple Myeloma) -1-3
构建方法: 来源于 Vk*MYC 转基因小鼠模型的自发性肿瘤，该小鼠携带受免疫球蛋白κ轻链基因增强子控制的 MYC 癌基因，可自发产生与人类多发性骨髓瘤高度相似的浆细胞肿瘤。Vk12598 是其中一个可移植克隆，通过连续传代在 C57BL/6 小鼠中维持 -5
形态特性: 浆细胞样形态，悬浮生长或轻微贴壁
生长特性: 悬浮生长
细胞标志物: CD138+， B220- -5
特性特征:

1. 耐药特性: 对硼替佐米 (Bortezomib) 耐药 -3-4

2. 移植模型: 可通过尾静脉注射或股骨内注射在免疫健全的 C57BL/6 小鼠中建立系统性多发性骨髓瘤模型 -2-7

3. 疾病特征: 移植后在小鼠血清中可检测到 M 蛋白 (单克隆免疫球蛋白)，脾脏中可见 CD138+ 肿瘤细胞浸润 -1-5

4. 体外培养: 在标准培养条件下难以长期扩增，需采用特殊培养条件（如 3D 悬滴培养、低氧、高密度及细胞因子组合）以维持细胞存活和功能 -5

5. 基因修饰: 携带 MYC 转基因，可通过 Cre-loxP 系统调控 -1

6. 生物发光/荧光: 可根据实验需要转导荧光素酶或 GFP 基因，用于活体成像追踪 -5-6

7. 研究应用: 广泛用于多发性骨髓瘤发病机制研究、耐药机制研究、免疫治疗评估、溶瘤病毒治疗、联合治疗策略开发、骨病变研究、PET 示踪剂评估等 -2-3-4
   培养基: BioVector® Vk12598 细胞专用完全培养基（推荐基础成分：RPMI-1640 培养基，添加 10-20% 胎牛血清、1% 青霉素-链霉素、2mM L-谷氨酰胺、1mM 丙酮酸钠，以及细胞因子组合如 IL-6 等）
   培养条件: 气相：95% 空气，5% 二氧化碳；温度：37°C；推荐低氧培养 (6.5% O₂) 以增强细胞存活 -5
   传代方法: 细胞对培养条件敏感，常规传代困难。推荐使用 BioVector® 专用培养体系，在 3D 悬滴或低氧条件下维持培养，每 2-3 天补充新鲜培养基 -5
   冻存液: BioVector® 细胞冻存液（推荐配方：90% 胎牛血清 + 10% DMSO）
   质量控制: 支原体检测：阴性；无菌检测：阴性；体内成瘤能力验证：阳性；M 蛋白检测：阳性
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1（需在二级生物安全柜内操作）
   仅供科研使用

English:
BioVector® Vk12598 Mouse Myeloma Cell Line Datasheet

Product Name: BioVector® Vk12598 Cells
Synonyms: BioVector® Vk12598 Myeloma Cells
Chinese Name: BioVector® Vk12598 小鼠骨髓瘤细胞系
English Name: BioVector® Vk12598 Mouse Myeloma Cell Line
Product Category: Cancer Cell Line; Multiple Myeloma Model
Species of Origin: Mouse (Mus musculus), C57BL/6 background -1-4
Tissue of Origin: Spleen (isolated from spleens of transplanted tumor-bearing mice) -1-5
Cell Type: Plasma cell -1
Disease: Multiple Myeloma -1-3
Derivation: Derived from spontaneous tumors arising in the Vk*MYC transgenic mouse model, which carries the MYC oncogene under control of the immunoglobulin kappa light chain gene enhancer and spontaneously develops plasma cell tumors highly similar to human multiple myeloma. Vk12598 is one transplantable clone maintained in C57BL/6 mice by serial passage -5
Morphology: Plasma cell-like morphology, suspension or weak adherent growth
Growth Characteristics: Suspension growth
Cell Markers: CD138+, B220- -5
Characteristics:

1. Drug Resistance: Resistant to Bortezomib -3-4

2. Transplant Model: Can establish systemic multiple myeloma in immunocompetent C57BL/6 mice via intravenous tail vein injection or intrafemoral injection -2-7

3. Disease Features: M-protein (monoclonal immunoglobulin) detectable in mouse serum after transplantation; CD138+ tumor cell infiltration observed in spleen -1-5

4. In Vitro Culture: Difficult to expand in standard culture conditions; requires specialized culture conditions (e.g., 3D hanging drop culture, hypoxia, high density, cytokine cocktail) to maintain cell viability and function -5

5. Genetic Modification: Carries MYC transgene that can be regulated by Cre-loxP system -1

6. Bioluminescence/Fluorescence: Can be transduced with luciferase or GFP genes for in vivo imaging tracking as needed -5-6

7. Research Applications: Widely used in multiple myeloma pathogenesis studies, drug resistance mechanism research, immunotherapy evaluation, oncolytic virus therapy, combination therapy strategy development, bone lesion studies, and PET tracer evaluation -2-3-4
   Culture Medium: BioVector® Complete Medium for Vk12598 Cells (Recommended composition: RPMI-1640 medium supplemented with 10-20% fetal bovine serum, 1% penicillin-streptomycin, 2mM L-glutamine, 1mM sodium pyruvate, and cytokine cocktail such as IL-6)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C; Hypoxic culture (6.5% O₂) recommended to enhance cell survival -5
   Subculturing Method: Cells are sensitive to culture conditions; conventional passaging is difficult. Recommended to use BioVector® Specialized Culture System and maintain under 3D hanging drop or hypoxic conditions; replenish with fresh medium every 2-3 days -5
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; In Vivo Tumorigenicity Validation: Positive; M-protein Detection: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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