pMC.BESPX BioVector®微环生产质粒 / Minicircle Producer Plasmd BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® pMC.BESPX 微环生产质粒 / Minicircle Producer Plasmid 数据说明书

产品名称: BioVector® pMC.BESPX 质粒
同义词: BioVector® pMC.BESPX-MCS2
中文名称: BioVector® pMC.BESPX 微环生产质粒
英文名称: BioVector® pMC.BESPX Minicircle Producer Plasmid
产品类别: 微环DNA载体；非病毒基因治疗载体
质粒类型: 微环生产质粒
用途: 用于生产不含细菌骨架序列的高纯度微环DNA；基因治疗；体外高效转染；持续转基因表达研究
来源信息: 由斯坦福大学陈志英博士构建并馈赠
载体大小: 4084 bp
复制子: ColE1
筛选标记: 卡那霉素 (Kanamycin)
生产系统: 与 BioVector® ZYCY10P3S2T 大肠杆菌菌株配合使用，该菌株表达诱导型ΦC31整合酶和I-SceI内切酶
生产原理:

1. 在L-阿拉伯糖诱导下，ΦC31整合酶介导attB和attP位点间的重组，产生微环DNA和骨架环

2. I-SceI内切酶切割骨架环上的I-SceI识别位点，使其降解

3. 微环DNA仅含真核表达盒，不含细菌骨架序列，大小仅为亲本质粒的约50%
   生产优势:

4. 操作简便，仅需温度改变和L-阿拉伯糖诱导5小时

5. 产量高，每1000 ml过夜培养物可产3.4-4.8 mg微环DNA

6. 纯度高，污染DNA仅占0.4%-1.5%

7. 成本低，与标准质粒制备相当
   特性特征:

8. 无细菌骨架: 微环DNA不含细菌复制子和抗性基因，避免了CpG基序引起的免疫反应

9. 高效表达: 由于尺寸减小，转染效率提高，转基因表达更高效、更持久

10. 安全性高: 无病毒序列，无整合风险，免疫原性低

11. 多克隆位点: 含有多克隆位点(MCS2)，便于插入目的基因

12. 可调控表达: 可插入组织特异性启动子实现靶向表达
    应用领域:

13. 基因治疗: EBV阳性鼻咽癌靶向治疗、肿瘤基因治疗

14. RNA干扰: 表达siRNA稳定抑制乙肝病毒复制

15. CRISPR基因编辑: 作为供体DNA用于CRISPaint基因标记

16. 蛋白生产: 哺乳动物细胞高效表达重组蛋白

17. 干细胞研究: 诱导多能干细胞的重编程和基因修饰
    相关载体:

18. BioVector® pMC.BESPX-MCV-HF (用于Merkel细胞病毒基因组扩增)

19. BioVector® pMC.BESPX-oriP (EBV阳性细胞靶向表达载体)

20. BioVector® pMC.BESPX-CMV-luci (荧光素酶报告基因载体)

21. BioVector® pMC-Dest-BsaI (Golden Gate克隆用微环载体)
    相关菌株: BioVector® ZYCY10P3S2T 大肠杆菌菌株
    质控载体: BioVector® pUC19 质粒
    宿主菌: BioVector® DH5α 化学感受态细胞（用于质粒扩增）；BioVector® ZYCY10P3S2T 感受态细胞（用于微环生产）
    质量控制: 测序验证：100%正确；无菌检测：阴性；酶切鉴定：符合预期条带；微环生产效率验证：阳性
    储存与运输: -20°C或干冰运输与保存
    仅供科研使用

English:
BioVector® pMC.BESPX Minicircle Producer Plasmid Datasheet

Product Name: BioVector® pMC.BESPX Plasmid
Synonyms: BioVector® pMC.BESPX-MCS2
Chinese Name: BioVector® pMC.BESPX 微环生产质粒
English Name: BioVector® pMC.BESPX Minicircle Producer Plasmid
Product Category: Minicircle DNA Vector; Non-viral Gene Therapy Vector
Plasmid Type: Minicircle Producer Plasmid
Purpose: Production of high-purity minicircle DNA devoid of bacterial backbone sequences; gene therapy; efficient in vitro transfection; sustained transgene expression studies
Source: Constructed and gifted by Dr. Zhiying Chen, Stanford University
Vector Size: 4084 bp
Replicon: ColE1
Selection Marker: Kanamycin
Production System: Used in conjunction with BioVector® ZYCY10P3S2T E. coli strain, which expresses inducible ΦC31 integrase and I-SceI endonuclease
Production Principle:

1. Upon L-arabinose induction, ΦC31 integrase mediates recombination between attB and attP sites, generating minicircle DNA and a backbone circle

2. I-SceI endonuclease cleaves the I-SceI recognition site on the backbone circle, leading to its degradation

3. Minicircle DNA contains only the eukaryotic expression cassette, devoid of bacterial backbone sequences, approximately 50% the size of the parent plasmid
   Production Advantages:

4. Simplified procedure, requiring only a temperature change and 5-hour L-arabinose induction

5. High yield: 3.4-4.8 mg of minicircle DNA per 1000 ml of overnight culture

6. High purity: contaminating DNA accounts for only 0.4%-1.5%

7. Low cost: comparable to standard plasmid preparation
   Characteristics:

8. Bacterial Backbone-Free: Minicircle DNA lacks bacterial replicon and antibiotic resistance genes, avoiding immune responses caused by CpG motifs

9. High Expression Efficiency: Due to reduced size, transfection efficiency is improved, and transgene expression is more efficient and persistent

10. High Safety: No viral sequences, no integration risk, low immunogenicity

11. Multiple Cloning Site: Contains MCS2 for convenient insertion of genes of interest

12. Regulatable Expression: Can accommodate tissue-specific promoters for targeted expression
    Applications:

13. Gene Therapy: EBV-positive nasopharyngeal carcinoma targeted therapy, cancer gene therapy

14. RNA Interference: Expression of siRNA for stable inhibition of hepatitis B virus replication

15. CRISPR Gene Editing: Donor DNA for CRISPaint gene tagging

16. Protein Production: High-efficiency expression of recombinant proteins in mammalian cells

17. Stem Cell Research: Reprogramming and genetic modification of induced pluripotent stem cells
    Related Vectors:

18. BioVector® pMC.BESPX-MCV-HF (for Merkel cell virus genome amplification)

19. BioVector® pMC.BESPX-oriP (targeted expression vector for EBV-positive cells)

20. BioVector® pMC.BESPX-CMV-luci (luciferase reporter vector)

21. BioVector® pMC-Dest-BsaI (minicircle vector for Golden Gate cloning)
    Related Strain: BioVector® ZYCY10P3S2T E. coli Strain
    Control Vector: BioVector® pUC19 Plasmid
    Host Strain: BioVector® DH5α Chemically Competent Cells (for plasmid amplification); BioVector® ZYCY10P3S2T Competent Cells (for minicircle production)
    Quality Control: Sequencing Validation: 100% correct; Sterility: Negative; Restriction Digestion: Expected bands; Minicircle Production Efficiency: Positive
    Storage and Shipment: Stored and shipped at -20°C or on dry ice
    For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net