BioVector® PIG3V人白癜风黑素细胞永生化细胞系Human Vitiligo Melanocyte Immortalized Cell Line BioVector NTCC保藏中心

BioVector® PIG3V 人白癜风黑素细胞永生化细胞系 / Human Vitiligo Melanocyte Immortalized Cell Line 数据说明书

产品名称: BioVector® PIG3V 细胞
中文名称: BioVector® PIG3V 人白癜风黑素细胞永生化细胞系
英文名称: BioVector® PIG3V Human Vitiligo Melanocyte Immortalized Cell Line
产品类别: 皮肤疾病研究细胞系
物种来源: 人 (Homo sapiens)
组织来源: 皮肤；表皮
细胞类型: 黑素细胞 (Melanocyte)
疾病: 白癜风 (Vitiligo)
患者信息: 白癜风供体 Ma9308P4
构建方法: 采用携带HPV16 E6和E7基因的逆转录病毒载体LXSN16E6E7转染白癜风患者来源的原代黑素细胞，经遗传霉素筛选和克隆纯化后建立
形态特性: 树突状黑素细胞形态
生长特性: 贴壁生长
特性特征:

1. 永生化特性: 已传代超过100次群体倍增，远超正常成人黑素细胞（最多50次倍增）

2. 白癜风特征保留: 超微结构显示扩大的内质网，这是白癜风黑素细胞的特征性结构

3. 蛋白质滞留: 扩大的内质网中存在分子量为37.5、47.5和56.5 kDa的蛋白质滞留

4. 黑色素生成: 酪氨酸酶加工正常，黑素体保持色素沉着，表型上更接近正常黑素细胞而非黑色素瘤细胞

5. miR-211表达缺失: PIG3V细胞中miR-211表达严重下调，而其靶基因PPARGC1A、RRM2和TAOK1表达上调

6. 线粒体功能缺陷: 线粒体复合物I、II和IV功能缺陷，呼吸反应异常，氧消耗率降低

7. 氧化应激: 活性氧产生增加，对氧化应激敏感

8. Nrf2信号: Nrf2信号通路受损，过表达Nrf2可促进线粒体生物合成和功能

9. 研究应用: 广泛用于白癜风发病机制研究、氧化应激研究、miRNA功能研究、线粒体功能障碍研究、自身抗原转运研究、药物筛选等
   培养基: BioVector® PIG3V 细胞专用完全培养基（推荐基础成分：Medium 254基础培养基，添加1% HMGS-2黑素细胞生长补充剂、10%胎牛血清和1%青霉素-链霉素）
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞融合度达到70-80%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA）进行消化，按1:2至1:4比例进行传代，每2-3天换液一次
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；HPV16 E6/E7基因检测：阳性；白癜风特征验证：内质网扩张
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1（需在二级生物安全柜内操作）
   仅供科研使用

English:
BioVector® PIG3V Human Vitiligo Melanocyte Immortalized Cell Line Datasheet

Product Name: BioVector® PIG3V Cells
Chinese Name: BioVector® PIG3V 人白癜风黑素细胞永生化细胞系
English Name: BioVector® PIG3V Human Vitiligo Melanocyte Immortalized Cell Line
Product Category: Skin Disease Research Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Skin; Epidermis
Cell Type: Melanocyte
Disease: Vitiligo
Patient Information: Vitiligo donor Ma9308P4
Derivation: Established by transfecting primary melanocytes derived from a vitiligo patient with the retroviral construct LXSN16E6E7 carrying HPV16 E6 and E7 genes, followed by geneticin selection and clonal purification
Morphology: Dendritic melanocyte morphology
Growth Characteristics: Adherent
Characteristics:

1. Immortalization Properties: Has undergone more than 100 population doublings since establishment, significantly exceeding the maximum of 50 doublings for normal adult melanocytes

2. Vitiligo Feature Retention: Ultrastructural characterization reveals dilated endoplasmic reticulum profiles, characteristic of vitiligo melanocytes

3. Protein Retention: Retention of proteins with molecular weights of 37.5, 47.5, and 56.5 kDa within the dilated endoplasmic reticulum

4. Melanogenesis: Tyrosinase is processed properly, melanosomes remain pigmented, and the phenotype resembles melanocytes rather than melanoma cells in culture

5. miR-211 Deficiency: PIG3V cells show severely downregulated miR-211 expression, with reciprocal upregulation of its target genes PPARGC1A, RRM2, and TAOK1

6. Mitochondrial Dysfunction: Defects in mitochondrial complexes I, II, and IV; abnormal respiratory responses; reduced oxygen consumption rate

7. Oxidative Stress: Increased reactive oxygen species production; sensitive to oxidative stress

8. Nrf2 Signaling: Impaired Nrf2 signaling pathway; overexpression of Nrf2 promotes mitochondrial biosynthesis and function

9. Research Applications: Widely used in vitiligo pathogenesis research, oxidative stress studies, miRNA function studies, mitochondrial dysfunction research, autoantigen transport studies, and drug screening
   Culture Medium: BioVector® Complete Medium for PIG3V Cells (Recommended composition: Medium 254 basal medium supplemented with 1% HMGS-2 melanocyte growth supplement, 10% fetal bovine serum, and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When cells reach 70-80% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:2 to 1:4. Medium renewal is recommended every 2-3 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; HPV16 E6/E7 Gene Detection: Positive; Vitiligo Feature Validation: Dilated endoplasmic reticulum
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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