BioVector® CW11033 人诱导多能干细胞系 / Human Induced Pluripotent Stem Cell Line BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® CW11033 人诱导多能干细胞系 / Human Induced Pluripotent Stem Cell Line 数据说明书

产品名称: BioVector® CW11033 细胞
同义词: BioVector® CIRMi12J-A
中文名称: BioVector® CW11033 人诱导多能干细胞系
英文名称: BioVector® CW11033 Human Induced Pluripotent Stem Cell Line
产品类别: 诱导多能干细胞(iPSC)系
物种来源: 人 (Homo sapiens)
组织来源: 外周血
细胞类型: T淋巴细胞来源的诱导多能干细胞
患者信息: 55岁女性；非裔美国人
疾病: 单相抑郁症
构建方法: 采用附加型载体(非整合型)重编程技术，从外周血T淋巴细胞建立

构建机构: 美国加州再生医学研究所
形态特性: 胚胎干细胞样形态，形成紧密的克隆集落，边界清晰
生长特性: 贴壁生长，依赖饲养层细胞或无饲养层培养体系
特性特征:

1. 多能性标志物: 表达多能性标志物OCT4、NANOG、LIN28、SSEA-4等

2. 分化潜能: 具有向三个胚层分化的潜能

3. 核型: 正常女性核型(46, XX)

4. 病毒检测: HIV-1、HIV-2、乙肝病毒、丙肝病毒检测均为阴性；支原体检测阴性

5. 研究应用: 疾病模型研究(抑郁症)、药物筛选、发育生物学研究、细胞治疗研究
   培养基: BioVector® CW11033 细胞专用完全培养基(推荐基础成分：Essential 8培养基或StemFit AK02N培养基)
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞集落达到适宜密度时，推荐使用 BioVector® 专用传代试剂(如EDTA或分散酶)进行消化，按适宜比例进行传代
   冻存液: BioVector® 干细胞专用冻存液
   质量控制: 支原体检测：阴性；无菌检测：阴性；多能性标志物表达：阳性；核型分析：正常
   储存与运输: 液氮或干冰运输与保存(-150°C至-190°C)
   生物安全等级: 1
   仅供科研使用

English:
BioVector® CW11033 Human Induced Pluripotent Stem Cell Line Datasheet

Product Name: BioVector® CW11033 Cells
Synonyms: BioVector® CIRMi12J-A
Chinese Name: BioVector® CW11033 人诱导多能干细胞系
English Name: BioVector® CW11033 Human Induced Pluripotent Stem Cell Line
Product Category: Induced Pluripotent Stem Cell (iPSC) Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Peripheral blood
Cell Type: T-lymphocyte-derived induced pluripotent stem cells
Patient Information: 55-year-old female; African American
Disease: Unipolar depression
Derivation: Established from peripheral blood T-lymphocytes using episomal vector (non-integrating) reprogramming technology
Establishing Institution: California Institute for Regenerative Medicine, USA
Morphology: Embryonic stem cell-like morphology, forming tight colonies with clear boundaries
Growth Characteristics: Adherent growth, dependent on feeder cells or feeder-free culture systems
Characteristics:

1. Pluripotency Markers: Expresses pluripotency markers OCT4, NANOG, LIN28, SSEA-4, etc.

2. Differentiation Potential: Capable of differentiating into three germ layers

3. Karyotype: Normal female karyotype (46, XX)

4. Virus Testing: Negative for HIV-1, HIV-2, Hepatitis B virus, Hepatitis C virus; Mycoplasma negative

5. Research Applications: Disease modeling (depression), drug screening, developmental biology research, cell therapy research
   Culture Medium: BioVector® Complete Medium for CW11033 Cells (Recommended composition: Essential 8 medium or StemFit AK02N medium)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When colonies reach appropriate density, recommended to use BioVector® Subculture Reagent (e.g., EDTA or dispase). Subculture at an appropriate split ratio
   Freeze Medium: BioVector® Stem Cell Freeze Medium
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Pluripotency Marker Expression: Positive; Karyotype Analysis: Normal
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1
   For Research Use Only

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