RN46A BioVector®大鼠神经嵴干细胞系 / Rat Neural Crest Stem Cell Line BioVector NTCC典型培养物保藏中心

BioVector® RN46A 大鼠神经嵴干细胞系 / Rat Neural Crest Stem Cell Line 数据说明书

产品名称: BioVector® RN46A 细胞
同义词: BioVector® RN-46A
中文名称: BioVector® RN46A 大鼠神经嵴干细胞系
英文名称: BioVector® RN46A Rat Neural Crest Stem Cell Line
产品类别: 神经干细胞系；条件永生化细胞系
物种来源: 大鼠 (Rattus norvegicus)，Sprague Dawley品系
组织来源: 胚胎第13天(E13)大鼠延髓中缝核
细胞类型: 神经嵴干细胞；血清素能神经元前体细胞
构建方法: 用编码温度敏感性SV40大T抗原(tsA58)的逆转录病毒感染胚胎第13天(E13)大鼠延髓中缝核细胞，经感染、克隆筛选建立
永生化机制: 在允许温度(33°C)下，SV40大T抗原表达，细胞增殖；在非允许温度(39°C)下，SV40大T抗原失活，细胞停止分裂并启动分化程序
形态特性: 增殖条件下呈成纤维细胞形态；分化条件下呈神经元形态，延伸神经突
生长特性: 贴壁生长；温度依赖性生长

特性特征:

1. 神经元限制性: 细胞系具有神经元限制性，分化后获得神经元表型

2. 分化潜能: 在39°C分化条件下，细胞停止分裂，呈现神经元形态，表达神经元特异性烯醇化酶和所有三种神经丝蛋白

3. 血清素能特性: 分化后表达色氨酸羟化酶；经脑源性神经营养因子处理和部分膜去极化条件(40mM KCl)诱导后，可合成血清素

4. 受体表达: 表达低亲和力神经生长因子受体和trkB受体；表达高亲和力5-HT1A受体，分化后Bmax增加20倍

5. 转运体: 表达钠依赖性5HT再摄取转运体；表达突触囊泡单胺转运体

6. 研究应用: 广泛用于血清素能神经元分化机制研究、神经丝组装调控研究、神经递质合成调控研究、神经发育生物学研究、慢性疼痛模型细胞移植治疗研究
   亚系: BioVector® RN46A-B14细胞(转染BDNF基因的自分泌BDNF分泌亚系)
   培养基: BioVector® RN46A 细胞专用完全培养基(推荐基础成分：DMEM:F12(1:1)培养基，添加10%胎牛血清，0.25mg/mL G418，2mM L-谷氨酰胺)
   培养条件: 气相：95%空气，5%二氧化碳；增殖培养温度：33°C；分化培养温度：39°C
   传代方法: 当细胞融合度达到70-80%时，推荐使用 BioVector® 专用传代试剂(如0.25%胰蛋白酶-EDTA)进行消化，按1:2至1:5比例进行传代，建议接种密度为2-4×10⁴细胞/cm²
   冻存液: BioVector® 细胞冻存液(推荐配方：90%胎牛血清 + 10% DMSO)
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；分化潜能验证：阳性
   储存与运输: 液氮或干冰运输与保存(-150°C至-190°C)
   生物安全等级: 1
   仅供科研使用

English:
BioVector® RN46A Rat Neural Crest Stem Cell Line Datasheet

Product Name: BioVector® RN46A Cells
Synonyms: BioVector® RN-46A
Chinese Name: BioVector® RN46A 大鼠神经嵴干细胞系
English Name: BioVector® RN46A Rat Neural Crest Stem Cell Line
Product Category: Neural Stem Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Rat (Rattus norvegicus), Sprague Dawley strain
Tissue of Origin: Embryonic day 13 (E13) rat medullary raphe nucleus
Cell Type: Neural crest stem cells; Serotonergic neuronal precursor cells
Derivation: Established by infecting embryonic day 13 (E13) rat medullary raphe cells with a retrovirus encoding the temperature-sensitive SV40 large T antigen (tsA58), followed by infection and clonal selection
Immortalization Mechanism: At the permissive temperature (33°C), SV40 large T antigen is expressed, allowing cell proliferation; at the non-permissive temperature (39°C), SV40 large T antigen is inactivated, cells cease division and initiate differentiation
Morphology: Fibroblast morphology under proliferating conditions; neuronal morphology with neurite extension under differentiating conditions
Growth Characteristics: Adherent; Temperature-dependent growth
Characteristics:

1. Neuronal Restriction: The cell line is neuronally restricted, acquiring neuronal phenotype upon differentiation

2. Differentiation Potential: Under differentiating conditions at 39°C, cells cease dividing, exhibit neuronal morphology, and express neuron-specific enolase and all three neurofilament proteins

3. Serotonergic Properties: Expresses tryptophan hydroxylase upon differentiation; synthesizes serotonin following brain-derived neurotrophic factor treatment and partial membrane depolarization conditions (40mM KCl)

4. Receptor Expression: Expresses low-affinity nerve growth factor receptor and trkB receptors; expresses high-affinity 5-HT1A receptors with 20-fold increase in Bmax upon differentiation

5. Transporters: Expresses sodium-dependent 5HT reuptake transporter; expresses synaptic vesicular monoamine transporter

6. Research Applications: Widely used in serotonergic neuronal differentiation mechanism studies, neurofilament assembly regulation research, neurotransmitter synthesis regulation studies, neurodevelopmental biology research, and cell transplantation therapy studies for chronic pain models
   Sublines: BioVector® RN46A-B14 Cells (autocrine BDNF-secreting subline transfected with BDNF gene)
   Culture Medium: BioVector® Complete Medium for RN46A Cells (Recommended composition: DMEM:F12 (1:1) medium supplemented with 10% fetal bovine serum, 0.25mg/mL G418, 2mM L-glutamine)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Proliferation Culture Temperature: 33°C; Differentiation Culture Temperature: 39°C
   Subculturing Method: When cells reach 70-80% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:2 to 1:5. Recommended seeding density is 2-4×10⁴ cells/cm²
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Differentiation Potential Validation: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1
   For Research Use Only

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