CIHP-1 BioVector®人肾脏足细胞系 / Human Kidney Podocyte Cell Line BioVector NTCC典型培养物保藏中心

BioVector® CIHP-1 人肾脏足细胞系 / Human Kidney Podocyte Cell Line 数据说明书

产品名称: BioVector® CIHP-1 细胞
同义词: BioVector® CIHP1
中文名称: BioVector® CIHP-1 人肾脏足细胞系
英文名称: BioVector® CIHP-1 Human Kidney Podocyte Cell Line
产品类别: 肾细胞系；条件永生化细胞系
物种来源: 人 (Homo sapiens)
组织来源: 肾脏；肾小球
细胞类型: 足细胞 (Podocyte)
构建方法: 通过温度敏感性SV40LT抗原转基因构建的条件永生化细胞系
永生化机制: 在许可温度（33°C）下，SV40LT抗原表达，细胞增殖；在非许可温度（37°C）下，SV40LT抗原失活，细胞停止分裂并启动分化程序，获得成熟足细胞表型
形态特性: 分化条件下呈现典型的足细胞形态，具有足突样结构
生长特性: 贴壁生长；温度依赖性生长
特征标志物: 表达成熟足细胞特异性标志蛋白，包括nephrin、podocin、CD2AP、synaptopodin
特性特征:

1. 分化潜能: 在37°C分化条件下，细胞获得与体内成熟足细胞高度相似的生理表型

2. 均质性: 提供均质、稳定、可长期培养的细胞来源

3. 研究应用: 广泛用于肾脏生理学、毒性学研究、代谢研究、药物靶向筛选、共培养模型（如3D培养）、糖尿病肾病、急性肾损伤、足细胞凋亡机制等研究领域

4. 文献支持: 已被全球超过100篇同行评审出版物引用，是国际公认的足细胞研究模型
   培养基: BioVector® CIHP-1 细胞专用完全培养基（推荐基础成分：RPMI-1640培养基，添加10%胎牛血清、1%胰岛素-转铁蛋白-硒、1%青霉素-链霉素）
   培养条件: 气相：95%空气，5%二氧化碳；增殖培养温度：33°C；分化培养温度：37°C
   传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA）进行消化，按1:3至1:6比例进行传代

冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
质量控制: 支原体检测：阴性；无菌检测：阴性；标志物表达验证：阳性
储存与运输: 液氮或干冰运输与保存
生物安全等级: 1（需在二级生物安全柜内操作）
仅供科研使用

English:
BioVector® CIHP-1 Human Kidney Podocyte Cell Line Datasheet

Product Name: BioVector® CIHP-1 Cells
Synonyms: BioVector® CIHP1
Chinese Name: BioVector® CIHP-1 人肾脏足细胞系
English Name: BioVector® CIHP-1 Human Kidney Podocyte Cell Line
Product Category: Kidney Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Kidney; Glomerulus
Cell Type: Podocyte
Derivation: Conditionally immortalized cell line generated using a temperature-sensitive SV40LT antigen transgene
Immortalization Mechanism: At the permissive temperature (33°C), the SV40LT antigen is expressed, allowing cell proliferation. At the non-permissive temperature (37°C), the SV40LT antigen is inactivated, cells cease division, and initiate a differentiation program, acquiring a mature podocyte phenotype
Morphology: Exhibits typical podocyte morphology upon differentiation, with foot process-like structures
Growth Characteristics: Adherent; Temperature-dependent growth
Marker Expression: Expresses mature podocyte-specific marker proteins, including nephrin, podocin, CD2AP, and synaptopodin
Characteristics:

1. Differentiation Potential: Under differentiating conditions at 37°C, cells acquire a physiological phenotype highly analogous to mature podocytes in vivo

2. Homogeneity: Provides a homogeneous, stable, and long-lasting cell source

3. Research Applications: Widely used in renal physiology, toxicology studies, metabolism research, drug targeting screening, co-culture models (e.g., 3D culture), diabetic nephropathy, acute kidney injury, and podocyte apoptosis mechanism studies

4. Literature Support: Cited in over 100 peer-reviewed publications worldwide; an internationally recognized model for podocyte research
   Culture Medium: BioVector® Complete Medium for CIHP-1 Cells (Recommended composition: RPMI-1640 medium supplemented with 10% fetal bovine serum, 1% Insulin-Transferrin-Selenium, 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Proliferation Culture Temperature: 33°C; Differentiation Culture Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:3 to 1:6
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Marker Expression Validation: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net