pYL-kasOp-FnCas12a2 BioVector® 基因编辑质粒 / pYL-kasOp-FnCas12a2 Plasmid

BioVector® pYL-kasOp-FnCas12a2基因编辑质粒 / pYL-kasOp-FnCas12a2 Plasmid 数据说明书

产品名称: BioVector® pYL-kasOp-FnCas12a2 质粒
中文名称: BioVector® pYL-kasOp-FnCas12a2 CRISPR基因编辑质粒
英文名称: BioVector® pYL-kasOp-FnCas12a2 CRISPR Gene Editing Plasmid
产品类别: CRISPR基因编辑质粒
质粒类型: CRISPR-Cas12a表达载体
用途: 表达链霉菌密码子优化的FnCas12a蛋白，用于链霉菌基因组修饰 -1
来源信息: 由Yunzi Luo实验室构建并保藏 -1
Addgene编号: 177874 -1
载体骨架: pCRISPomyces-2 -1
插入片段: 链霉菌密码子优化的FnCas12a基因 (3909 bp) -1

别名: FnCas12a, FnCpf1 -1
启动子: kasOp* -1
筛选标记:

• 细菌筛选：安普霉素 (Apramycin, 25 μg/mL) -1

• 真核筛选：URA3 -1
  复制子: 高拷贝 -1
  克隆方法: Gibson Assembly -1
  测序引物:

• 5′ 测序引物: ACCGAACAGGCCATTCACAGA -1

• 3′ 测序引物: TCTGACGCTCAGTGGAACGA -1
  细菌培养条件:

• 推荐菌株: NEB Stable -1

• 培养温度: 37°C -1

• 抗性: 安普霉素 (25 μg/mL) -1
  相关质粒: BioVector® pYL-kasOp-FnCas12a3 (FnCas12a突变体EP16) -2
  质控载体: BioVector® pUC19 质粒
  宿主菌: BioVector® DH5α 化学感受态细胞
  质量控制: 测序验证：100%正确；无菌检测：阴性
  储存与运输: -20°C或干冰运输与保存
  仅供科研使用

English:
BioVector® pYL-kasOp-FnCas12a2 Plasmid Datasheet

Product Name: BioVector® pYL-kasOp-FnCas12a2 Plasmid
Chinese Name: BioVector® pYL-kasOp-FnCas12a2 CRISPR基因编辑质粒
English Name: BioVector® pYL-kasOp-FnCas12a2 CRISPR Gene Editing Plasmid
Product Category: CRISPR Gene Editing Plasmid
Plasmid Type: CRISPR-Cas12a Expression Vector
Purpose: Expresses Streptomyces codon-optimized FnCas12a for genome modification in Streptomyces -1
Source: Constructed and deposited by the Yunzi Luo Lab -1
Addgene ID: 177874 -1
Vector Backbone: pCRISPomyces-2 -1
Insert: Streptomyces codon-optimized FnCas12a gene (3909 bp) -1
Synonyms: FnCas12a, FnCpf1 -1
Promoter: kasOp* -1
Selection Markers:

• Bacterial Selection: Apramycin (25 μg/mL) -1

• Eukaryotic Selection: URA3 -1
  Replicon: High Copy Number -1
  Cloning Method: Gibson Assembly -1
  Sequencing Primers:

• 5′ Sequencing Primer: ACCGAACAGGCCATTCACAGA -1

• 3′ Sequencing Primer: TCTGACGCTCAGTGGAACGA -1
  Bacterial Growth Conditions:

• Recommended Strain: NEB Stable -1

• Growth Temperature: 37°C -1

• Resistance: Apramycin (25 μg/mL) -1
  Related Plasmid: BioVector® pYL-kasOp-FnCas12a3 (FnCas12a mutant EP16) -2
  Control Vector: BioVector® pUC19 Plasmid
  Host Strain: BioVector® DH5α Chemically Competent Cells
  Quality Control: Sequencing Validation: 100% correct; Sterility: Negative
  Storage and Shipment: Stored and shipped at -20°C or on dry ice
  For Research Use Only

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