EML BioVector® 小鼠红系-髓系-淋巴系造血祖细胞系 / Mouse Erythroid-Myeloid-Lymphoid Hematopoietic Progenitor Cell Line

BioVector® EML 小鼠红系-髓系-淋巴系造血祖细胞系 / Mouse Erythroid-Myeloid-Lymphoid Hematopoietic Progenitor Cell Line 数据说明书

产品名称: BioVector® EML 细胞
同义词: BioVector® EML Cell Line, Clone 1; BioVector® EML C1
中文名称: BioVector® EML 小鼠红系-髓系-淋巴系造血祖细胞系
英文名称: BioVector® EML Mouse Erythroid-Myeloid-Lymphoid Hematopoietic Progenitor Cell Line
产品类别: 造血干细胞/祖细胞模型
物种来源: 小鼠 (Mus musculus)，BDF1品系，雄性 -3
组织来源: 骨髓 -1-3
细胞类型: 多能造血祖细胞
构建方法: 用表达显性负效维甲酸受体 (dnRARα) 的逆转录病毒感染经5-氟尿嘧啶处理的小鼠骨髓细胞，随后在含有干细胞因子的培养基中培养获得 -1-10
保藏编号: ATCC CRL-3610 -3
形态特性: 不规则细胞样，悬浮生长 -2-3
生长特性: 悬浮生长，对培养条件敏感
特性特征:

1. 多向分化潜能: 在体外可诱导分化为红系、髓系和淋巴系细胞 -1-4-5

2. 功能异质性: 细胞群根据CD34表达可分为不同亚群：

   • Sca-1+ c-kit+ Flk- CD34+ 细胞（约占80%）和 Sca-1+ c-kit+ Flk- CD34- 细胞（约占20%） -5-7

   • CD34+ 细胞在SCF刺激下生长，包含大部分具有多系分化潜能的祖细胞 -1-5

   • CD34- 细胞在IL-3刺激下生长，分化能力较弱，可能代表更原始的"静息"状态细胞 -1-5

   • CD34+ 细胞群本身也具有异质性，CD34高表达细胞产生集落能力最强，CD34低表达细胞具有回复为CD34-状态的能力 -5-7

3. 自我更新: 可在含SCF的培养基中无限增殖，并可重复克隆而不失去多能性 -1

4. 研究应用: 被公认为研究造血早期分化事件、CD34表达调控、造血干细胞激活机制以及造血相关基因功能的理想体外模型 -1-4-5
   培养基: BioVector® EML 细胞专用完全培养基（推荐基础成分：Iscove's改良DMEM培养基，添加20%胎牛血清和200 ng/mL小鼠干细胞因子） -3
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C -3
   传代方法: 维持细胞密度在4×10⁵ - 2×10⁶活细胞/mL之间，每2-3天添加新鲜培养基或更换培养基 -3
   冻存液: BioVector® 细胞冻存液（推荐配方：完全生长培养基添加5% DMSO） -3
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；多系分化潜能验证：阳性
   储存与运输: 液氮气相或干冰运输与保存（-130°C以下） -3
   生物安全等级: 1
   仅供科研使用

English:
BioVector® EML Mouse Erythroid-Myeloid-Lymphoid Hematopoietic Progenitor Cell Line Datasheet

Product Name: BioVector® EML Cells
Synonyms: BioVector® EML Cell Line, Clone 1; BioVector® EML C1
Chinese Name: BioVector® EML 小鼠红系-髓系-淋巴系造血祖细胞系
English Name: BioVector® EML Mouse Erythroid-Myeloid-Lymphoid Hematopoietic Progenitor Cell Line
Product Category: Hematopoietic Stem/Progenitor Cell Model
Species of Origin: Mouse (Mus musculus), BDF1 strain, Male -3
Tissue of Origin: Bone marrow -1-3
Cell Type: Multipotent hematopoietic progenitor cells
Derivation: Established by infecting bone marrow cells from 5-fluorouracil-treated mice with a retrovirus expressing a dominant-negative retinoic acid receptor (dnRARα), followed by culture in medium containing stem cell factor -1-10
Deposit Number: ATCC CRL-3610 -3
Morphology: Irregular cell-like, suspension growth -2-3
Growth Characteristics: Suspension growth, sensitive to culture conditions
Characteristics:

1. Multilineage Differentiation Potential: Can be induced to differentiate into erythroid, myeloid, and lymphoid lineages in vitro -1-4-5

2. Functional Heterogeneity: The cell population can be separated into subpopulations based on CD34 expression:

   • Sca-1+ c-kit+ Flk- CD34+ cells (approximately 80%) and Sca-1+ c-kit+ Flk- CD34- cells (approximately 20%) -5-7

   • CD34+ cells proliferate in response to SCF and contain the majority of progenitors capable of multilineage differentiation -1-5

   • CD34- cells proliferate in response to IL-3, show poor differentiation capacity, and may represent a more primitive "quiescent" state -1-5

   • The CD34+ population is also heterogeneous: CD34high cells have the highest colony-forming capacity, while CD34low cells retain the ability to revert to a CD34- state -5-7

3. Self-Renewal: Can be propagated indefinitely in SCF-containing medium and recloned without losing multipotentiality -1

4. Research Applications: Recognized as an ideal in vitro model for studying early hematopoietic differentiation events, CD34 expression regulation, hematopoietic stem cell activation mechanisms, and gene function in hematopoiesis -1-4-5
   Culture Medium: BioVector® Complete Medium for EML Cells (Recommended composition: Iscove's modified Dulbecco's medium supplemented with 20% fetal bovine serum and 200 ng/mL mouse stem cell factor) -3
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C -3
   Subculturing Method: Maintain cell density between 4×10⁵ - 2×10⁶ viable cells/mL; add fresh medium or replace medium every 2-3 days -3
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: Complete growth medium supplemented with 5% DMSO) -3
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Multilineage Differentiation Potential: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen vapor phase or on dry ice (below -130°C) -3
   Biosafety Level: 1
   For Research Use Only

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