HPC-7 BioVector®小鼠造血祖细胞系 / Mouse Hematopoietic Progenitor Cell Line

BioVector® HPC-7 小鼠造血祖细胞系 / Mouse Hematopoietic Progenitor Cell Line 数据说明书

产品名称: BioVector® HPC-7 细胞
同义词: BioVector® HPC7
中文名称: BioVector® HPC-7 小鼠造血祖细胞系
英文名称: BioVector® HPC-7 Mouse Hematopoietic Progenitor Cell Line
产品类别: 血液细胞系；造血干细胞/祖细胞模型
物种来源: 小鼠 (Mus musculus) -1-4
组织来源: 胚胎干细胞 -2-4
细胞类型: 多能造血祖细胞 -1-2-3
构建方法: 通过逆转录病毒在胚胎干细胞中表达LHx2基因，经体外分化筛选获得 -2-4
形态特性: 悬浮生长，圆形

生长特性: 悬浮生长，对培养条件敏感 -2
标志物表达:

• 阳性标志物: c-kit+， CD34+， CD44+ -4

• 阴性标志物: Sca-1-， Thy1.2- -4

• 转录因子: 表达与造血干细胞特征一致的转录因子 -4
  特性特征:

1. 造血重建能力: 注射到致死辐照宿主后可完全重建造血系统 -9

2. 分化潜能: 通过添加不同细胞因子可诱导分化，分化后细胞表面标志物表达发生变化 -4

3. 自我更新: 保留造血干细胞特征性的自我更新能力 -4

4. 研究应用: 被公认为造血干细胞/祖细胞（HSPC）的细胞系模型，已生成与ENCODE tier 1细胞系相当的基因组规模数据资源 -3

5. 粘附分子: 表达与原代造血干细胞一致的粘附分子，可用于研究造血干细胞归巢机制 -5-6-9
   培养基: BioVector® HPC-7 细胞专用完全培养基（推荐基础成分：IMDM培养基，添加10%胎牛血清，10% SCF条件培养基，1% L-谷氨酰胺，1%青霉素-链霉素和74.8μM单硫代甘油） -1
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C -1
   传代方法: 维持细胞密度在5×10⁵ - 2×10⁶ cells/mL之间，每2-3天传代或补加新鲜培养基 -1。解冻后至少可培养10代而不显著影响细胞标志物表达和功能 -4
   冻存液: BioVector® 细胞冻存液
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；物种鉴定：小鼠来源已验证 -4；病原体检测：小鼠必需病原体检测阴性 -4
   储存与运输: 液氮或干冰运输与保存（-196°C） -4
   生物安全等级: 1
   仅供科研使用

English:
BioVector® HPC-7 Mouse Hematopoietic Progenitor Cell Line Datasheet

Product Name: BioVector® HPC-7 Cells
Synonyms: BioVector® HPC7
Chinese Name: BioVector® HPC-7 小鼠造血祖细胞系
English Name: BioVector® HPC-7 Mouse Hematopoietic Progenitor Cell Line
Product Category: Blood Cell Line; Hematopoietic Stem/Progenitor Cell Model
Species of Origin: Mouse (Mus musculus) -1-4
Tissue of Origin: Embryonic stem cells -2-4
Cell Type: Multipotent hematopoietic progenitor cells -1-2-3
Derivation: Generated by retrovirally expressing the LHx2 gene in embryonic stem cells, followed by in vitro differentiation selection -2-4
Morphology: Suspension, round
Growth Characteristics: Suspension growth, sensitive to culture conditions -2
Marker Expression:

• Positive Markers: c-kit+, CD34+, CD44+ -4

• Negative Markers: Sca-1-, Thy1.2- -4

• Transcription Factors: Expresses transcription factors consistent with characterized HSCs -4
  Characteristics:

1. Hematopoietic Reconstitution Capacity: Can fully reconstitute hematopoiesis when injected into a lethally irradiated host -9

2. Differentiation Potential: Can be differentiated through the addition of different cell factors; cell-surface expression changes post-differentiation -4

3. Self-Renewal: Retains the self-renewal properties characteristic of HSCs -4

4. Research Applications: Recognized as a cell line model for hematopoietic stem/progenitor cells (HSPCs); genome-scale data resources comparable to ENCODE tier 1 cell lines have been generated -3

5. Adhesion Molecules: Expresses adhesion molecules known to be present on primary HSCs; used to study HSC homing mechanisms -5-6-9
   Culture Medium: BioVector® Complete Medium for HPC-7 Cells (Recommended composition: IMDM supplemented with 10% fetal calf serum, 10% SCF conditioned media, 1% L-Glutamine, 1% penicillin/streptomycin, and 74.8μM monothioglycerol) -1
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C -1
   Subculturing Method: Maintain cell density between 5×10⁵ - 2×10⁶ cells/mL; subculture or replenish medium every 2-3 days -1. Can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality -4
   Freeze Medium: BioVector® Cell Freeze Medium
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Species Verification: Confirmed as mouse origin -4; Pathogen Testing: Negative for mouse essential pathogens -4
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-196°C) -4
   Biosafety Level: 1
   For Research Use Only

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