pEAQ-HT-DEST1 BioVector®植物高水平双元瞬时表达载体Plasmid BioVector NTCC典型培养物保藏中心

Product Name / 产品名称:
BioVector® pEAQ-HT-DEST1 Plasmid植物双元表达载体，专为在植物中进行重组蛋白的高水平瞬时表达而设计
BioVector® pEAQ-HT-DEST1质粒

Description / 描述:
The BioVector® pEAQ-HT-DEST1 plasmid is a binary plant expression vector designed for high-level transient expression of recombinant proteins in plants -1. It combines virus-derived sequences to enhance translation efficiency with a Gateway® recombination cassette for facile cloning -1-4. Developed from the pEAQ (Easy and Quick) vector series, this plasmid enables rapid production of recombinant proteins at yields up to milligrams per gram of fresh leaf mass upon agroinfiltration -1. The vector is widely used for molecular pharming, protein subcellular localization studies, and functional characterization of plant and pathogen proteins -1-8.
BioVector® pEAQ-HT-DEST1 质粒是一种植物双元表达载体，专为在植物中进行重组蛋白的高水平瞬时表达而设计 -1。它结合了病毒来源的序列以增强翻译效率，并包含Gateway®重组盒以便于克隆 -1-4。该质粒源自pEAQ载体系列，通过农杆菌浸润法可在植物中实现高达毫克级/克鲜重的重组蛋白产量 -1。该载体广泛应用于分子农业、蛋白质亚细胞定位研究以及植物和病原体蛋白的功能鉴定 -1-8。

Original Source / 原始来源:
Developed by the Lomonossoff Lab, John Innes Centre, UK / 由英国约翰英纳斯中心Lomonossoff实验室开发

Key Publications / 关键文献:
Sainsbury F, et al. pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol J. 2009;7(7):682-693.

Peyret H, Lomonossoff GP. The pEAQ vector series: the easy and quick way to produce recombinant proteins in plants. Plant Mol Biol. 2013;83(1-2):51-58.

Plasmid Type / 质粒类型:

• Binary plant expression vector / 植物双元表达载体

• Gateway® destination vector / Gateway®目的载体

• Transient expression vector / 瞬时表达载体

Species / 物种:

• Agrobacterium tumefaciens (for plant transformation) / 根癌农杆菌（用于植物转化）

• Escherichia coli (for propagation) / 大肠杆菌（用于扩增）

• Plant hosts (especially Nicotiana benthamiana) / 植物宿主（特别是本氏烟）

Vector Backbone / 载体骨架:
pEAQ-HT-derived / pEAQ-HT衍生载体

Total Vector Size / 总载体大小:
~10.2 kb (10,247 bp) -2 / 约10.2 kb

Key Components / 关键组件:

Gene/Insert Details / 基因/插入片段详情:

Growth Conditions in Bacteria / 细菌生长条件:

Applications / 应用:

• High-level Transient Expression / 高水平瞬时表达: Produces recombinant proteins at yields up to mg/g fresh weight in N. benthamiana-1

• Molecular Pharming / 分子农业: Production of therapeutic proteins, vaccines, and industrial enzymes -1

• Protein Subcellular Localization / 蛋白质亚细胞定位: Used as backbone for fluorescent fusion protein vectors -1-4

• Effector Biology / 效应子生物学: Expression of pathogen effectors to study plant-pathogen interactions -8

• Metabolic Engineering / 代谢工程: Reconstruction of biosynthetic pathways in plants -2-6

• Virus-like Particle Production / 病毒样颗粒生产: Production of vaccine candidates and VLPs

Derived Vector Series / 衍生载体系列:
A collection of 15 pEAQ-HT-DEST1-based vectors has been developed for protein subcellular localization studies -1-4:

Quality Control / 质量控制:
Plasmid is verified by restriction digestion and sequencing. Full sequence information is available from public databases (NCBI accession: 257196397) -2.
质粒经过限制性酶切和测序验证。完整序列信息可从公共数据库获取。

Storage / 存储:

• Plasmid is sent as a bacterial stab in agar or as purified DNA. Upon receipt, streak onto a plate with BioVector® Kanamycin (50 μg/mL), grow a single colony, and prepare a glycerol stock for long-term storage at -80°C.

• 质粒以细菌穿刺菌或纯化DNA形式提供。收到后，应在含 BioVector® 卡那霉素 (50 μg/mL) 的平板上划线，挑取单克隆，并制备甘油菌于-80°C长期保存。

Plasmid Reconstitution Protocol / 质粒溶解复配方案:

1. Centrifuge at 5,000×g for 5 min. / 5,000×g离心5分钟。

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. / 小心打开管盖，加入20 μl无菌水溶解DNA。

3. Close the tube and incubate for 10 minutes at room temperature. / 盖紧管盖，室温孵育10分钟。

4. Briefly vortex and spin down. / 短暂涡旋并离心收集。

5. Store the plasmid at -20°C. / 于-20°C保存质粒。

6. Important Note / 重要提示: The intact pEAQ-HT-DEST1 plasmid contains the ccdB gene and must be propagated in E. coli strains resistant to ccdB effects (e.g., DB3.1). For expression clones, use standard cloning strains after Gateway® recombination. / 完整的pEAQ-HT-DEST1质粒含有ccdB基因，必须在抗ccdB效应的大肠杆菌菌株（如DB3.1）中扩增。对于表达克隆，在Gateway®重组后使用标准克隆菌株。

Citation / 引用:
When using this plasmid, please cite the following publications:
使用此质粒时，请引用以下文献：

Sainsbury F, Thuenemann EC, Lomonossoff GP. pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol J. 2009;7(7):682-693.

For derived fluorescent protein vectors, also cite:
Berthold F, et al. Inside or outside? A new collection of Gateway vectors allowing plant protein subcellular localization or over-expression. Plasmid. 2019;105:102436.

Important Note / 重要提示:

• The pEAQ-HT-DEST1 plasmid is designed for Agrobacterium-mediated transient expression in plants, particularly Nicotiana benthamiana-1-8

• pEAQ-HT-DEST1质粒专为农杆菌介导的植物瞬时表达设计，特别适用于本氏烟 -1-8

• The vector contains virus-derived sequences (CPMV UTRs) that dramatically enhance translation efficiency without viral replication -1

• 该载体含有病毒来源的序列，可在无病毒复制的情况下显著增强翻译效率 -1

• The P19 silencing suppressor is encoded within the T-DNA to prevent post-transcriptional gene silencing and maximize expression -1-2

• P19沉默抑制子编码在T-DNA内，可防止转录后基因沉默，最大化表达水平 -1-2

• The Gateway® cassette enables rapid, directional cloning of genes of interest without restriction enzymes -1-4

• Gateway®盒可实现目的基因的快速、定向克隆，无需限制性内切酶 -1-4

• This vector has been successfully used in multiple plant species including pea, lentil, and faba bean, though efficiency varies -8

• 该载体已成功用于多种植物物种，包括豌豆、扁豆和蚕豆，但效率因物种而异 -8

BioVector® Kanamycin
BioVector® 卡那霉素

For bacterial selection of the pEAQ-HT-DEST1 plasmid and its derivatives.
用于pEAQ-HT-DEST1质粒及其衍生质粒的细菌筛选。

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址http://www.biovector.net