BioVector® pUB110 枯草芽孢杆菌广宿主载体 (Bacillus subtilis Broad-host-range Vector)

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BioVector® pUB110 (枯草芽孢杆菌载体)

载体类型：枯草芽孢杆菌 (Bacillus subtilis) 表达/克隆载体。
来源：最初分离自金黄色葡萄球菌 (Staphylococcus aureus)，是研究最广泛的滚环复制 (RCR) 质粒之一。
全长： 4546 bp。
复制子：pUB110 复制起点（支持在枯草芽孢杆菌及多种革兰氏阳性菌中高拷贝复制）。
抗性标记：Neomycin (新霉素) 或 Kanamycin (卡那霉素) 抗性基因 (kan/neo)。
主要特征：
- 稳定性：在没有选择压力的情况下，在枯草芽孢杆菌中具有中等的偏析稳定性。
- 宿主范围：广泛的宿主兼容性，包括 Bacillus, Staphylococcus, and Actinomycetes。
- 结构：包含复制启动蛋白编码基因 (repU) 和单链复制起点 (ssOir)。
主要应用：枯草芽孢杆菌基因表达系统的构建、蛋白质分泌研究、工业酶制剂的生产以及作为构建穿梭载体 (Shuttle vector) 的基础骨架。

Figure 1 from Plasmid rolling circle replication and its control. | Semantic Scholar

BioVector® pUB110 (Bacillus subtilis Broad-host-range Vector)

Vector Type: Bacillus subtilis Expression/Cloning Vector.
Origin: Originally isolated from Staphylococcus aureus; one of the most thoroughly characterized rolling-circle replicating (RCR) plasmids.
Length: 4546 bp.
Replicon:pUB110 origin (supports high-copy replication in B. subtilis and other Gram-positive bacteria).
Selection Marker:Neomycin or Kanamycin resistance基因 (kan/neo).
Key Features:
- Stability: Exhibits moderate segregational stability in B. subtilis without active selection.
- Host Range: Exceptionally broad, compatible with Bacillus, Staphylococcus, and certain Actinomycetes.
- Architecture: Encodes the replication initiation protein (repU) and the single-strand origin (ssOir).
Applications: Construction of B. subtilis expression systems, protein secretion research, industrial enzyme production, and as a scaffold for developing Gram-positive/Gram-negative shuttle vectors.