HPB-Ma/LTR-FL/CMV-RL细胞Cell Line BioVector NTCC®细胞保藏中心
- 价 格：¥99850
- 货 号：HPB-Ma/LTR-FL/CMV-RL
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
[Designation名称] NTCC® Synonyms: HPB-Ma/LTR-FL/CMV-RL Cell Line细胞株
BioVector NTCC Inc.Synonyms细胞别名: Cell Line Name: X4-MaRBLEComments: Population: Japanese.NTCC® Accession Number: CVCL_E8AVReferences identifiers: PubMed=17182760;Comments: Characteristics: Reporter cell line to measure HIV-1 infectivity and thus evaluate susceptibility to anti-HIV-1 drugs (PubMed=17182760).Comments: Characteristics: Transfected with two constructs. 1) 53LTRN-lucneoR#1 which contains the HIV-1 strain HXB2 LTR driving the expression of a Luc-DsRed fusion protein. This allows HIV-1 replication to be quantitatively evaluated by using luciferase activity when the LTR is activated by Tat, and the DsRed gene allows HIV-1 infected cells to be identified by red fluorescence. 2) pRenillaPac where Renilla luciferase is under the control of a CMV promoter and is used to monitor cell numbers and viability (PubMed=17182760).Comments: Virology: Infected with Moloney murine leukemia virus (MoMuLV) from MiCl1 (S+L-) (Cellosaurus=CVCL_3405) cells (from parent cell line).Comments: Virology: Susceptible to infection by CXCR4-tropic HIV-1 strains (example HXB2) (PubMed=17182760).Comments: Sequence variation: Gene fusion; HGNC; HGNC:1539; CBFB + HGNC; HGNC:29824; MYL11; Name(s)=CBFB-MYL11, CBFB-MYLPF (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1574Pro (c.4721T>C); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Asp2442fs*39 (c.7326_7326insGGGCCGTGGACG); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys124Ter (c.372C>A); ClinVar=VCV000458537; Zygosity=Homozygous (from parent cell line).Comments: Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E8AV
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: HPB-Ma/LTR-FL/CMV-RL Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
BioVector NTCC Inc.Synonyms细胞别名: Cell Line Name: X4-MaRBLEComments: Population: Japanese.NTCC® Accession Number: CVCL_E8AVReferences identifiers: PubMed=17182760;Comments: Characteristics: Reporter cell line to measure HIV-1 infectivity and thus evaluate susceptibility to anti-HIV-1 drugs (PubMed=17182760).Comments: Characteristics: Transfected with two constructs. 1) 53LTRN-lucneoR#1 which contains the HIV-1 strain HXB2 LTR driving the expression of a Luc-DsRed fusion protein. This allows HIV-1 replication to be quantitatively evaluated by using luciferase activity when the LTR is activated by Tat, and the DsRed gene allows HIV-1 infected cells to be identified by red fluorescence. 2) pRenillaPac where Renilla luciferase is under the control of a CMV promoter and is used to monitor cell numbers and viability (PubMed=17182760).Comments: Virology: Infected with Moloney murine leukemia virus (MoMuLV) from MiCl1 (S+L-) (Cellosaurus=CVCL_3405) cells (from parent cell line).Comments: Virology: Susceptible to infection by CXCR4-tropic HIV-1 strains (example HXB2) (PubMed=17182760).Comments: Sequence variation: Gene fusion; HGNC; HGNC:1539; CBFB + HGNC; HGNC:29824; MYL11; Name(s)=CBFB-MYL11, CBFB-MYLPF (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1574Pro (c.4721T>C); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Asp2442fs*39 (c.7326_7326insGGGCCGTGGACG); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys124Ter (c.372C>A); ClinVar=VCV000458537; Zygosity=Homozygous (from parent cell line).Comments: Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E8AV
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: HPB-Ma/LTR-FL/CMV-RL Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
- 公告/新闻
