BIRe003-A-3细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Synonyms: BIRe003-A-3 Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: Cell Line Name: UMANe002-A-3
Cross-references: hPSCreg; UMANe002-A-3
NTCC® Accession Number: CVCL_E3RR
Cross-references: BioSamples; SAMEA114526381
References identifiers: PubMed=38460234;
Comments: From: University of Manchester; Manchester; United Kingdom.
Comments: Population: Caucasian.
Comments: Characteristics: Produces a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling (from parent cell line).
Comments: Characteristics: Using CRISPR/Cas9 a T2A-dTomato construct was integrated at the 3'end of both alleles of HAND1 (PubMed=38460234).
Comments: Characteristics: Using CRISPR/Cas9 a T2A-eGFP construct was integrated at the 3'end of one allele of NKX2-5 (from parent cell line).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Comments: Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Comments: Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E3RR
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[Reference参考文献]
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培养方法：
Medium培养基：NTCC® Synonyms: BIRe003-A-3 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net