FIRE2FLPCS-520-8细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® FIRE2FLIPCS clone 1 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: FIRE2FLPCS-520-8
References identifiers: PubMed=39824193;
Comments: Characteristics: Expresses a Fe-IRE (FIRE) biosensor that senses IRP activity and cellular iron status (PubMed=39824193).
Comments: Characteristics: This cell line contains a FIRE2 biosensor (emiRFP670-sensor, mRuby3-control) and is transposed with two PiggyBac constructs FIRES-FLIP-PBCS-E670 and FIREC-PBCS-MR3 flanking FIRE sensor and control fluorescent protein, respectively. The FLIP-FIRE sensor is composed of a 740 bp sequence synthesized in backwards orientation and based on known mammalian IRP1/2 binding sites (PubMed=39824193).
Comments: Genetic integration: Method=PiggyBac transposition; Gene=FPbase; YHJV5; emiRFP670 (Note=Constitutively fluorescent near IR fluoresent protein from R.palustris).
Comments: Genetic integration: Method=PiggyBac transposition; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611).
Comments: Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Comments: Cell type: Embryonic stem cell; CL=CL_0002322.
Comments: Breed/subspecies: C57BL/6J x 129S4/SvJae.
Species of origin: NCBI_TaxID=10090; ! Mus musculus (Mouse)
Hierarchy: CVCL_C865 ! v6.5
Sex of cell: Male
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_E6HT
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® FIRE2FLIPCS clone 1 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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