ES2 / Luciferase & GFP细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® ES2/Luciferase-2A-GFP Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: ES2 / Luciferase & GFP
Web pages: Provider; GenTarget; SC066-LG; https://www.gentarget.com/product/human-es2-luciferase-gfp-stable-cells/
Comments: Problematic cell line: Probably misclassified. Parent cell line seems to originate from a Caucasian patient with an ovarian serous adenocarcinoma origin.
Comments: Population: Caucasian.
Comments: Characteristics: Transduced by lentivirus with a EF1a-Luc-2A-GFP-Rsv-PuroR-WPRE construct (GenTarget=SC066-LG).
Comments: Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
Comments: Sequence variation: Mutation; HGNC; HGNC:26144; PALB2; Simple; p.Lys295_Met296insTer (c.886delA) (p.Met296Terfs); ClinVar=VCV000143979; Zygosity=Unspecified (from parent cell line).
Comments: Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.242_243CC>TT (-138/-139CC>TT); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Ser241Phe (c.722C>T); ClinVar=VCV000012359; Zygosity=Homozygous (from parent cell line).
Comments: Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_E8YF
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ES2/Luciferase-2A-GFP Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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