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[Designation名称] NTCC® KJL-Luc Cell Line细胞株
BioVector NTCC Inc.Synonyms细胞别名: ID iLite TNF-alpha Assay Ready CellsWeb pages: Provider; Svar Life Science; BM3044; https://www.svarlifescience.com/products/bm3044Accession Number: NTCC® CVCL_E7RUReferences identifiers: PubMed=21910993;Comments: Population: Caucasian.Comments: Characteristics: Genetically engineered and optimized to respond to TNF-alpha resulting in the specific expression of firefly luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla luciferase reporter gene construct, under control of a constitutive promoter (Svar Life Science=BM3044).Comments: Characteristics: The luciferase reporter gene is under the control of 5 tandem copies of the NF-kappaB response element and the Renilla luciferase is under the control of a constitutive minimal TK promoter (PubMed=21910993).Comments: Sequence variation: Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.Comments: Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.Diseases: C9110; Blast phase chronic myelogenous leukemia, BCR-ABL1 positive
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_E7RU
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法:
Medium培养基:NTCC® KJL-Luc Complete Medium
Growth properties形态:贴壁/悬浮:
Culture Condition培养条件:Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址:www.biovector.net
BioVector NTCC Inc.Synonyms细胞别名: ID iLite TNF-alpha Assay Ready CellsWeb pages: Provider; Svar Life Science; BM3044; https://www.svarlifescience.com/products/bm3044Accession Number: NTCC® CVCL_E7RUReferences identifiers: PubMed=21910993;Comments: Population: Caucasian.Comments: Characteristics: Genetically engineered and optimized to respond to TNF-alpha resulting in the specific expression of firefly luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla luciferase reporter gene construct, under control of a constitutive promoter (Svar Life Science=BM3044).Comments: Characteristics: The luciferase reporter gene is under the control of 5 tandem copies of the NF-kappaB response element and the Renilla luciferase is under the control of a constitutive minimal TK promoter (PubMed=21910993).Comments: Sequence variation: Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.Comments: Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.Diseases: C9110; Blast phase chronic myelogenous leukemia, BCR-ABL1 positive
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_E7RU
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法:
Medium培养基:NTCC® KJL-Luc Complete Medium
Growth properties形态:贴壁/悬浮:
Culture Condition培养条件:Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址:www.biovector.net
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