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[Designation名称] NTCC® PD57-7 MT8 Cell Line细胞株
BioVector NTCC Inc.Synonyms细胞别名: ID ICGi052-B-1Cross-references: hPSCreg; ICGi052-B-1Accession Number: NTCC® CVCL_E9B9Cross-references: BioSamples; SAMEA117400231References identifiers: PubMed=40149527;Comments: From: The Federal Research Center Institute of Cytology and Genetics; Novosibirsk; Russia.Comments: Population: Caucasian.Comments: Characteristics: Upon doxycycline induction expresses a MitoTimer biosensor. MitoTimer is a DsRed-Timer protein fused to the transit peptide of COX18 so as to target it to the mitochondrial matrix (PubMed=40149527; DOI=10.3390/biomedicines13030550; PMCID=PMC11940372).Comments: Characteristics: Using CRISPR/Cas9 a Neo-M2rtTA construct and a Puro-TRE-mCMV-MitoTimer were introduced at the AAVS1 safe harbor locus (PubMed=40149527).Comments: Sequence variation: Mutation; HGNC; HGNC:6893; MAPT; Simple; p.Asn279Lys (c.837T>G) (N596K, c.1788T>G or N614K, c.1842T>G); ClinVar=VCV000014253; Zygosity=Heterozygous (PubMed=40149527).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 55DQE; DsRed-Timer.Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_E9B9
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法:
Medium培养基:NTCC® PD57-7 MT8 Complete Medium
Growth properties形态:贴壁/悬浮:
Culture Condition培养条件:Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址:www.biovector.net
BioVector NTCC Inc.Synonyms细胞别名: ID ICGi052-B-1Cross-references: hPSCreg; ICGi052-B-1Accession Number: NTCC® CVCL_E9B9Cross-references: BioSamples; SAMEA117400231References identifiers: PubMed=40149527;Comments: From: The Federal Research Center Institute of Cytology and Genetics; Novosibirsk; Russia.Comments: Population: Caucasian.Comments: Characteristics: Upon doxycycline induction expresses a MitoTimer biosensor. MitoTimer is a DsRed-Timer protein fused to the transit peptide of COX18 so as to target it to the mitochondrial matrix (PubMed=40149527; DOI=10.3390/biomedicines13030550; PMCID=PMC11940372).Comments: Characteristics: Using CRISPR/Cas9 a Neo-M2rtTA construct and a Puro-TRE-mCMV-MitoTimer were introduced at the AAVS1 safe harbor locus (PubMed=40149527).Comments: Sequence variation: Mutation; HGNC; HGNC:6893; MAPT; Simple; p.Asn279Lys (c.837T>G) (N596K, c.1788T>G or N614K, c.1842T>G); ClinVar=VCV000014253; Zygosity=Heterozygous (PubMed=40149527).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 55DQE; DsRed-Timer.Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_E9B9
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法:
Medium培养基:NTCC® PD57-7 MT8 Complete Medium
Growth properties形态:贴壁/悬浮:
Culture Condition培养条件:Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址:www.biovector.net
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