HT809-GBA1(L444P/L444P) + GPNMB KO细胞株NTCC® Cell Line BioVector细胞保藏中心

[Designation名称] NTCC® HT809-GBA1(L444P/L444P) + GPNMB KO Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: ID HT809-TH-tdTomato-LysoIP-MitoIP-GBA1 L444P/L444P + GPNMB KO
Comments: Population: Jewish; Ashkenazi.
Accession Number: NTCC® CVCL_F1L4
References identifiers: PubMed=40667145;
Comments: Characteristics: Using CRISPR/Cas9 a P2-tdTomato constructs was inserted at the C-terminus of one allele of TH. This allows dopaminergic neurons differentiated from this cell line to express tdTomato and be readily identified (from parent cell line).
Comments: Characteristics: Using TALEN a CAG driven construct containing TMEM192-mGFP-3xHA (LysoIP tag) and TOM20-mTagBFP-TwinStrep(2 copies of WSHPQFEK) (MitoIP tag) separated by a T2A self-cleavage peptide was inserted at one allele of the CLYBL locus (from parent cell line).
Comments: Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:4462; GPNMB.
Comments: Sequence variation: Mutation; HGNC; HGNC:4177; GBA1; Simple_corrected; p.Asn409Ser (c.1226A>G) (N370S); ClinVar=VCV000004290; Zygosity=Homozygous; Note=By CRISPR/Cas9 (PubMed=40667145).
Comments: Sequence variation: Mutation; HGNC; HGNC:4177; GBA1; Simple_edited; p.Leu483Pro (c.1448T>C) (L444P); ClinVar=VCV000004288; Zygosity=Homozygous; Note=By CRISPR/Cas9 (PubMed=40667145).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Comments: Genetic integration: Method=TALEN; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments: Genetic integration: Method=TALEN; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
Comments: Genetic integration: Method=TALEN; Gene=HGNC; HGNC:26775; TMEM192.
Comments: Genetic integration: Method=TALEN; Gene=HGNC; HGNC:20947; TOM20.
Comments: Derived from site: In situ; Skin; UBERON=UBERON_0002097.
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[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_F1L4
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[Reference参考文献]
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培养方法：
Medium培养基：NTCC® HT809-GBA1(L444P/L444P) + GPNMB KO Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net