HL5; Human PRLR-Luciferase clone 5细胞株NTCC® Cell Line BioVector细胞保藏中心

[Designation名称] NTCC® HL5; Human PRLR-Luciferase clone 5 Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: ID HEK293 HL5
References identifiers: PubMed=12824168;
Accession Number: NTCC® CVCL_E7I6
References identifiers: PubMed=12668884;
Web pages: Provider; Inserm Transfert; RT00002; https://technology-offers.inserm-transfert.com/offer/hek-293-cells-expressing-human-prolactin-receptor-and-lhre-lucifease-reporter-gene/
Comments: Characteristics: Used in a transcriptional bioassay for lactogens. Upon binding a lactogen to PRLR a complex is generated that activates JAK2 which in turn activates STAT5 which results in transcriptional activation of the luciferase reporter gene. Thus, the luciferase activity reflects receptor activation by the exogenous PRLR ligand (PubMed=12668884).
Comments: Characteristics: Transfected with two constructs, one containing PRLR under the control of a CMV promoter and the other a firefly luciferase reporter gene under the control of the HSV-TK promoter containing a six-repeat lactogenic hormone response element (LHRE) (PubMed=12668884).
Comments: Genetic integration: Method=Transfection; Gene=HGNC; HGNC:9446; PRLR.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Comments: Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Comments: Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: CVCL_0045 ! HEK293
Sex of cell: Female
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[Accession编号] NTCC®-Accession Number: NTCC® CVCL_E7I6
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培养方法：
Medium培养基：NTCC® HL5; Human PRLR-Luciferase clone 5 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net